How does a PD-10 column work?
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
What is desalting in protein purification?
Desalting is used to remove salts from protein solutions, phenol or unincorporated nucleotides from nucleic acids or excess crosslinking or labeling reagents from conjugated proteins.
How do PD 10 columns work?
What are the disposable PD-10 desalting columns made of?
Disposable PD-10 Desalting Columns From GE Healthcare. The PD-10 columns are made of chemically inert polypropylene material and filled with 8.5 mL of Sephadex-25 gel filtration material. The exclusion limit of the Sephadex-25 is 5000 for globular protein. The column can be fitted with the LabMate buffer reservoir, which is very useful when
What is the recovery capacity of the PD 10 column?
The PD 10 column features fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium. Easy to use and equilibrate; apply sample; elute Desalt multiple samples in parallel with a desalting capacity >90% Recovery from 70 to >95%
What kind of preservative is in a PD 10 column?
The PD 10 column features fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium. Columns are packed in distilled water containing 0.15% Kathon CG as a preservative. Columns feature a polypropylene barrel and polyethylene frits.
What kind of gel is used in PD-10 columns?
The PD-10 columns are made of chemically inert polypropylene material and filled with 8.5 mL of Sephadex-25 gel filtration material. The exclusion limit of the Sephadex-25 is 5000 for globular protein.