What is ODS in hplc?
Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. We also offer hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.
What is the structure of an ODS column?
An ODS column is filled with a packing of octadecylsilyl groups (ODS groups or C18 groups) chemically bonded to a silica gel carrier. ODS columns are used for reverse-phase chromatography. ODS columns find a wide range of applications due to their high theoretical plate number and rapid equilibration.
How does C18 column work?
C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances. Knowing the characteristics of the compounds that will be run through the C18 columns can help you choose which one will work best for you.
What is the difference between BDS and ODS HPLC columns?
ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.
Which is the best packing material for HPLC?
Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety.
How big is the Shim pack XR-ODS column?
The Shim-pack XR-ODS column has a 2.2 µm particle size packing which will greatly shorten the analysis time by up to 1/5th that of a general-purpose column (5 µm particle size) while maintaining the resolution. The Shim-pack XR-C8, Phenyl, and SIL use similar 2.2 µm column packing material like the Shim-pack XR-ODS.
How many types of ODS columns are there?
Chemical structures differ slightly among solutes Hydrophobicity differs greatly among solutes Difficult to separate using standard ODS High molecular weight peptides and oligonucleic acids Guides for selecting C18 packing materials YMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics.
How are ODS groups used in reverse phase chromatography?
An ODS column is filled with a packing of octadecylsilyl groups (ODS groups or C18 groups) chemically bonded to a silica gel carrier. ODS columns are used for reverse-phase chromatography. ODS columns find a wide range of applications due to their high theoretical plate number and rapid equilibration.