What are HFOB cells?
We found that undifferentiated hFOB cells possess similar cell surface markers as BMMSCs and also express the embryonic stem cell-related pluripotency gene, Oct-4, as well as the neural progenitor marker nestin. This human cell line has been a widely used model of normal osteoblast differentiation.
What are MC3T3 E1 cells?
The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro.
What is MG 63 cells?
MG-63 cells, a line derived from an osteosarcoma, produce high yields of interferon after superinduction with polyinosinic acid polycytidylic acid, cycloheximide, and actinomycin D. Studies using MG-63 cells provide some important mechanistic clues concerning the details of the amplification process in tumors.
How do osteoblasts form new bone?
Osteoblasts work in teams to build bone. They produce new bone called “osteoid” which is made of bone collagen and other protein. Then they control calcium and mineral deposition. Some of the osteoblasts turn into osteocytes while the new bone is being formed, and the osteocytes then get surrounded by new bone.
What do osteoblasts cells do?
OSTEOBLASTS are the cells that form new bone. They produce new bone called “osteoid” which is made of bone collagen and other protein. Then they control calcium and mineral deposition. They are found on the surface of the new bone.
What is the function of osteocytes cells?
The potential functions of osteocytes include: to respond to mechanical strain and to send signals of bone formation or bone resorption to the bone surface, to modify their microenvironment, and to regulate both local and systemic mineral homeostasis.
What is the role of osteoblasts in bone remodeling?
The primary role of osteoblasts is to lay down new bone during skeletal development and remodelling. Through these mechanisms, cells of the osteoblast lineage help retain the homeostatic balance between bone formation and bone resorption.
Are osteocytes mature or immature cells?
When the area surrounding an osteoblast calcifies, the osteoblast becomes trapped and transforms into an osteocyte, the most common and mature type of bone cell.
Do osteocytes have a Nucleoid?
The cell body varies in size from 5-20 micrometers in diameter and contain 40-60 cell processes per cell, with a cell to cell distance between 20-30 micrometers. A mature osteocyte contains a single nucleus that is located toward the vascular side and has one or two nucleoli and a membrane.
What is the main function of an osteoblast?
Osteoblasts are specialized mesenchymal cells that synthesize bone matrix and coordinate the mineralization of the skeleton. These cells work in harmony with osteoclasts, which resorb bone, in a continuous cycle that occurs throughout life.
What is the function of osteoblast cells?
Osteoblasts work in teams to build bone. They produce new bone called “osteoid” which is made of bone collagen and other protein. Then they control calcium and mineral deposition. They are found on the surface of the new bone.