What is normal goat serum used for?
blocking agent
Normal goat serum (NGS) can be used as an effective blocking agent in a number of immunological techniques since it can successfully reduce nonspecific binding of proteins and antibodies to reaction surfaces and non-specific binding sites. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk.
How do you dilute normal goat serum?
Product Specific Information For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum. Restoration and Storage: Store product at 4°C until opened.
What is donkey serum?
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample. Serum can be used directly for blocking, or as a constituent of a blocking buffer.
What is normal horse serum?
Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species.
When should goats use serum?
Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
Why is BSA used for blocking?
BSA blocking is a routine practice among clinicians and researchers working on immunoassays throughout the world. The primary role of BSA is to prevent the non-specific binding by blocking the leftover spaces over solid surface after immobilization of a capture biomolecule.
What is Bovi sera for goats?
Bovi Sera is an absolute MUST HAVE for any goat owner. Useful in the prevention and treatment of conditions such as pneumonia and enterotoxemia, passive immune failure in newborns and shipping fever complex in adults, Bova Sera provides an immediate boost to the immune system allowing goats to get back on their feet.
What does a blocking buffer do?
Blocking buffers are used in various applications to decrease non-specific signaling generated by non-specific binding of proteins or peptides, essentially blocking Western blot noise.
How do you dilute a donkey serum?
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
What is the function of the blocking agents non fat milk and serum?
Types of blocking buffers
| Blocking Buffer/Agent | Benefits |
|---|---|
| Purified proteins (e.g., casein) | Single-protein blocking buffers can provide fewer chances of cross-reaction with assay components than serum or milk solutions. Ideal when blockers, such as non-fat milk, block antigen-antibody binding |
How do you store horse serum?
To effectively preserve the integrity of animal serum, it should be stored frozen and protected from light. For stability and optimal performance, serum should be stored at -10 to -40 C and used prior to the labeled expiration date.
What is goat serum made of?
Product FAQs S-1000, is essentially spun whole blood, filtered, heat inactivated and preserved with the addition of sodium azide. The 20 ml provided therefore is all goat serum.
How to measure the antifade properties of Vectashield mountants?
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.
What should the refractive index of Vectashield be?
If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light. The refractive index for VECTASHIELD Mounting Medium is 1.45.
Do you need to dehydrate tissue with Vectashield?
No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD.
What can normal goat serum be used for?
2.5% Normal Goat Serum can be used for blocking non-specific binding or as an antibody diluent. I recently purchased Normal Goat Serum, Cat. No. S-1000, from Vector Laboratories for use as a general block in my tissue staining applications.