Is LIN28 a transcription factor?
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28.
Is LIN28A real?
LIN28A (Lin-28 Homolog A) is a Protein Coding gene. Diseases associated with LIN28A include Embryonal Tumor With Multilayered Rosettes and Central Nervous System Primitive Neuroectodermal Neoplasm. Among its related pathways are Developmental Biology and Translational Control.
What is LIN protein?
About LIN Protein: LIN28 encodes an RNA-binding protein, which binds to and promotes translation of the IGF-2 (insulin-like growth factor 2) mRNA. LIN protein is found in ribosomes, P-bodies, and stress granules in pluripotent embryonal carcinoma cells.
What does LIN28 stand for?
Lin-28 homolog A is a protein that in humans is encoded by the LIN28 gene. LIN28 encodes an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA.
What does it mean that the HSCs are lineage Lin negative?
(2 points) Lineage-negative means that the HSCs do not express a panel of protein surface markers present on mature blood lineage cells.
What is Nanog gene?
NANOG (Nanog Homeobox) is a Protein Coding gene. Diseases associated with NANOG include Teratocarcinoma and Germ Cell And Embryonal Cancer. Among its related pathways are Mesodermal Commitment Pathway and Oct4 in Mammalian ESC Pluripotency.
What is Lin in flow cytometry?
Lineage Negative (Lin-) Markers Note A select list of Lin- markers are provided as examples of markers that have been used in the literature to exclude particular lineages of cells in order to help identify the cell type of interest.
What are LT HSCs?
Multipotent long-term HSCs (LT-HSCs) reside in the bone marrow and through a process of asymmetric cell division, can self-renew to sustain the stem cell pool or differentiate into short-term HSCs (ST-HSCs) or lineage-restricted progenitors that undergo extensive proliferation and differentiation to produce terminally …
What is the function of MYC?
MYC gene encodes a multifunctional, nuclear phosphoprotein that controls a variety of cellular functions, including cell cycle, cell growth, apoptosis, cellular metabolism and biosynthesis, adhesion, and mitochondrial biogenesis.
What is the function of Oct4?
Oct4 mainly functions through the activation of pluripotency-associated and self-renewal-associated genes, while simultaneously repressing genes that promote differentiation, in coordination with other pluripotency factors and coregulators.
What is the function of the LIN28 gene?
Lin-28 homolog A is a protein that in humans is encoded by the LIN28 gene. LIN28 encodes an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA.
How is LIN28 expressed in mouse embryos?
In mice, LIN28 is highly expressed in mouse embryonic stem cells and during early embryogenesis. LIN28 is highly expressed in human embryonic stem cells and can enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts.
How is LIN28 related to let-7 Precursor microRNAs?
“Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.” Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, MUTAGENESIS OF HIS-147 AND HIS-169. “TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation.”
How does LIN28 bind to IGF-2 mRNA?
LIN28 encodes an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28 binds to the let-7 pre-microRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells.