What is DiI staining?
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells.
What is CM Dil?
CellTracker™ CM-DiI is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the red excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.
What is a lipophilic dye?
Lipophilic carbocyanine dyes are cyanine fluorescent dyes with hydrophobic hydrocarbon tails. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers, making them useful for labeling membranes.
Did excitation and emission?
DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths….Product Attributes.
| CAS number | 127274-91-3 |
|---|---|
| Colors | Far-red |
| Excitation/Emission | 644/663 nm |
How does DiI labeling work?
DiI is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayer. The intense fluorescence and high photostability of the dye make it possible to visualize the fine structures (axons and dendrites) of the neurons.
Why do we need to label the membrane?
The labeling method could help researchers track membrane proteins in living animals. When a scientist labels a membrane protein with a dye, she has to wash the cells a few times to remove dye molecules that aren’t bound to the protein.
What is the difference between excitation and emission spectra?
An emission spectrum describes the wavelengths of the spectrum emitted by an energetic object. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit wavelengths of light, the emission spectrum2.
DiD far red?
The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is supplied as a solid form to facilitate direct application of crystals to membranes and is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.
What histological staining technique might you use to visualize dendritic spines?
Golgi-staining would be one of the best methods used for the in-depth analysis of dendritic spines.
What molecules are transported by aquaporins?
Which type of molecules are transported by aquaporins? Water, Aquaporins are channels in the membrane that allow water to pass through via osmosis.
What kind of light is DII C12 excited by?
1. DiI-C12 is a fluorescent dye which labels the liquid-disordered phase of a lipid membrane and is excited maximally by 549 nm light. Other fluorescent dyes which selectively partition into either the L o or the L d phase may be used.
What’s the difference between Dio and DII dyes?
DiOC18(3) [DiO] (3,3´ -dioctadecyloxacarbocyanine, perchlorate) is a widely used fluorescent membrane dye. However, DiO has been fluorescent emission and the lateral diffusion rate on the membranes is generally slower than that of DiI. DiO and DiI are often used together in dual color studies.
How is Dii used as a tracer in cells?
It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (V-22885), as a paste (N-22880) or as a solid (D-282).
Can a cm-DII dye be used for GFP?
Additionally, the excitation and emission spectra of CellTracker™ CM-DiI dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing. For Research Use Only. Not for use in diagnostic procedures.