What is LC-MS MS testing?

What is LC-MS MS testing?

Liquid chromatography mass spectrometry (LC-MS) is an analytical chemistry laboratory technique for identification, quantitation and mass analysis of materials. Minimal (or no) fragmentation occurs, allowing for determination of the molecule’s molecular weight.

What is LC-MS MS vs LC-MS?

LC-MS instruments are basically HPLC units with a mass spectrometry detector attached to it whereas LC-MS/MS is HPLC with two mass spectrometry detectors.

What is LC-MS MS technology?

Liquid chromatography–mass spectrometry (LC–MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS).

How much does a LC-MS cost?

As a rough approximation metals analyses usually run between $25 and $75 per sample, and LC/MS/MS and GC/MS/MS analyses are typically between $100 and $200 per sample. For lipidomics, the cost for running a quantitative analysis (targeted analysis of known lipids) is $120 per sample.

How is LC-MS MS done?

1) In LC/MS, the effluent from the LC is ionized, and the ions are directed into a mass spectrometer. The spectrometer generally records all ions within the set range. You can collect all ions within the working range and plot the total ion chromatograph (TIC). You can perform MS/MS however.

Is LC-MS expensive?

LC-MS is an expensive option, both in terms of capital and running costs. The instrument requires skilled personnel to set it up. However, after training, it is relatively easy to operate on a daily basis.

Is mass spectrometry expensive?

Mass spectrometry (MS) in clinical laboratories has a reputation for being both time intensive and costly.

How much is the sample for LC-MS?

1 mg sample is enough for LC-MS analysis… Actually with LCMS you need very few amount of sample. The concentration you will work with is between 1 to 10mg/L and the injected volume is generally 10microL. The real amount needed is ca 1 micro g.

How much does an LC-MS cost?

What is LC-MS used for?

The LC-MS/MS is a powerful tool for the detection of residual chemical compounds, confirmatory identification of small organic molecules, and confirmation and quantitation of contaminants and adulterants in pharmaceutical and food samples—for example, vanilla.