How do I select an Amicon?

How do I select an Amicon?

HOW TO SELECT AN AMICON® ULTRA FILTER

1. Determine the starting volume of your sample.
2. Determine the molecular weight (MW) of your macromolecule of interest, then choose an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule.
3. Select pack size.

What is Amicon filter?

Overview. Amicon® Ultra centrifugal filters provide fast sample processing and promote high sample recoveries, even in dilute samples, through ultrafiltration. The unique features of the Amicon® Ultra centrifugal filters give you the fastest, most efficient concentration for sensitive downstream applications.

How do you use centricon?

Centricon Plus-70 membranes contain trace amounts of glycerine, used as a humectant. To remove glycerine, fill device with 70 mL of buffer solution or Milli-Q® water and spin for 5 minutes. To remove all water and buffer, a recovery spin is required. Do not allow the membrane in the filter device to dry out once wet.

How does Amicon concentrate protein?

Attach the included Amicon® Ultra 0.5 mL filter (five MWCO options available) to the exchange device. Add 1.5 mL* of sample to the exchange device Spin 4000 x g for 15 min to concentrate. Add 1.5 mL* of desired buffer. Spin 4000 x g for 15 min to exchange buffer and concentrate.

How do you reuse an Amicon filter?

As Ammara mentioned, yes they can be reused. We reuse the filters for the same protein(same sample if working with cell lysates). After each use, we wash with milli Q , then 70% ethanol and finally with milli Q. We store them at 4 degree with milli Q or the buffer of your protein.

What are Centricons?

Centricon centrifugal filter devices provide fast, efficient concentra- tion and desalting of macromolecular solutions by ultrafiltration us- ing Millipore’s Ultracel® YM regenerated cellulose membranes. Centrifugal force drives solvents and low molecular weight solutes through the membrane into the filtrate vial.

How do you concentrate protein?

Proteins can be concentrated by precipitation from solution with ammonium sulfate, polyethylene glycol, organic solvent, trichloroacetic acid, potassium chloride/sodium dodecyl sulfate, and three-phase partitioning.

How do you concentrate protein after dialysis?

You make a concentrated suspension of high molecular weight PEG and put the dialysis tubing containing your sample in the suspension. The water is gradually drawn out into the suspension, concentrating the sample. Another way to do this is to cover the dialysis tubing with dry carboxymethylcellulose.

How do you remove salt from a protein solution?

Desalting is used to remove salts from protein solutions, phenol or unincorporated nucleotides from nucleic acids or excess crosslinking or labeling reagents from conjugated proteins.

What kind of filter is Amicon ultra 0.5 mL?

With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times for protein purification.

How are microcentrifuge tubes used in Amicon ultra 0.5?

The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

What are the spin conditions for Amicon ultra?

Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12.