What does reverse phase mean in HPLC?
Reversed-Phase HPLC The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface.
Why is C18 nonpolar?
In other terms, C18 has Octadecyl chains which are usually hydrophobic and highly retain nonpolar compounds. The length of its carbon chain is longer. Its lower hydrophobicity will cause faster retention of nonpolar compounds. Therefore, nonpolar compounds will move down the column more rapidly with C8 than with C18.
What is C18?
C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances.
Is C18 reverse phase?
A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.
Why is C18 used?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 HPLC columns are used in environmental sciences and chemical analysis, as well as industries such as pharmaceutical and environmental sciences, to analyze individual parts of chemical mixtures.
What is a C18 column?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances.
Why are reversed phase columns used in HPLC?
YMC America’s reversed phase columns are available in a full line of chemistries to optimize separations and provide improved retention and reliable selectivity. Reversed phase columns are one of the most common modes in HPLC analysis. Please explore all of our different products listed below:
Which is the primary mechanism of reversed-phase column selectivity?
C18 Reversed-phase Column Hydrophobicity is the primary mechanism of analyte interaction with C18 and other alkyl-bonded stationary phases. In addition, the polarity of the phase will also contribute to the overall selectivity observed.
What do you call a C18 HPLC column?
Also referred to as ODs or reversed-Phase C18 Columns. This is a separation system that uses high performance liquid chromatography system (HPLC) with C18 as the main stationary phase substance. It is important to note that single stationary C18 phases will always be different from one C18 HPLC to the other.
Why are reversed phase columns used in YMC America?
Reversed Phase Columns. Determined to maximize your productivity and flexibility in method development? YMC America’s reversed phase columns are available in a full line of chemistries to optimize separations and provide improved retention and reliable selectivity. Reversed phase columns are one of the most common modes in HPLC analysis.