What color is FITC?

What color is FITC?

FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. The color of the compound is yellow while the emitted light is green.

What is FITC used for?

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Is FITC hydrophobic?

FITC (fluorescein isothiocyanate) conjugated with dextran has a molecular weight of 4000–40,000 Da and, for sodium fluorescein, the molecular weight is around 376 Da (Wilhelm et al., 2011). Both of these compounds show fluorescence and are hydrophilic in nature; the excitation wavelength is between 490 and 520 nm.

Is FITC a stain?

for the labeling of proteins This staining dye “Fluorescein-5-isothiocyanate (FITC) – for biochemistry and for fluorescence microscopy” is used for human-medical cell diagnosis and serves the purpose of the cytological investigation of sample material of human origin.

Which fluorophores are brightest?

It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.

Does FITC overlap APC?

5 or APC). 2)Move CD62L to a detector that is still relatively bright, but does not overlap with FITC (such as APC or PE-Cy5). Note that in this example, only the CD8+ cells are highly stained in the FITC detector, so only CD8+ cells contribute to data spread in the PE detector.

Does PE bleed into FITC?

where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed.

Is FITC a dye?

1 Fluorescein. Fluorescein isothiocyanate (FITC) is the most widely used fluorescent probe for the preparation of conjugates with biological molecules (Hansen, 1967; Haugland, 1990). This xanthene dye (Fig.