What is meant by AFB staining?
AFB smear—a microscopic examination of a person’s sputum or other specimen that is stained to detect acid-fast bacteria. It is a rapid test used to provide presumptive results within one to two days.
What is the full meaning of AFB?
Acid-fast bacillus (AFB) is a type of bacteria that causes tuberculosis and certain other infections. Tuberculosis, commonly known as TB, is a serious bacterial infection that mainly affects the lungs.
Why we do AFB stain?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
What stain is used for AFB?
Auramine-O is a hydrochloride dye that causes stained AFB to emit fluorescence (green or yellow) when viewed under a fluorescence microscope. Unlike the Ziehl-Neelsen method, heating is not required for the penetration of the stain into the bacteria.
Why is TB acid-fast?
Acid-Fast Bacteria—Ziehl– Neelsen Stain This stain is used to identify Mycobacterium tuberculosis, the causative agent of tuberculosis. Acid-fast organisms have a lipoid capsule that has a high molecular weight and is waxy at room temperature. This makes the organism impenetrable by aqueous-based staining solutions.
Is TB curable in India?
Is this disease curable? Ans. Yes. However the patient has to take regular and full course of treatment, as advised by the doctor.
What does AFB mean military?
Alphabetical list of military acronyms and terms
| Acronym or Term | Meaning or Definition |
|---|---|
| A | Alpha |
| AFB | Air Force Base |
| AWOL | Absent Without Leave |
| AFRC | Air Force Reserve Command |
How is AFB culture done?
To do an AFB culture, healthcare providers take a sample of phlegm or sputum you’re coughing up or a tiny bit of your tissue. They “culture” it by putting it in a special container with food the bacteria needs to grow. They then check it over a few weeks’ time to see whether the bacteria grow.
Is TB acid-fast?
Sputum, or phlegm, is often used to test for Mycobacterium tuberculosis, to find out if a patient has TB. This bacterium is completely acid-fast, which means the entire cell holds onto the dye.
How do you stain AFB?
Procedure of Acid-Fast Stain
- Prepare bacterial smear on clean and grease free slide, using sterile technique.
- Allow smear to air dry and then heat fix.
- Cover the smear with carbol fuchsin stain.
- Heat the stain until vapour just begins to rise (i.e. about 60 C).
- Wash off the stain with clean water.
What stains acid-fast?
The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat.
What is sputum for AFB?
A sputum stain for Mycobacteria is a laboratory test performed on a sample of your sputum, or phlegm. It’s also known as an acid-fast bacillus (AFB) stain or a tuberculosis (TB) smear. A doctor typically orders the test to determine if a person has tuberculosis (TB) or another type of mycobacterial infection.
What is the process of AFB staining?
A typical AFB stain procedure involves dropping the cells in suspension onto a slide, then air drying the liquid and heat fixing the cells. Studies have shown that an AFB stain without a culture has a poor negative predictive value. An AFB culture should be performed along with an AFB stain; this has a much higher negative predictive value.
What is the process of acid fast staining?
Procedure of Acid-Fast Stain Prepare bacterial smear on clean and grease free slide, using sterile technique. Allow smear to air dry and then heat fix. Cover the smear with carbol fuchsin stain. Heat the stain until vapour just begins to rise (i.e. about 60 ). Wash off the stain with clean water.
How does acid fast staining work?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses. Your health care provider will collect a sample of urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection.
What is modified acid fast?
modified acid-fast stain a stain for coccidia ( Cryptosporidium , Cyclospora, Isospora ) in which the decolorizer is a highly acid (1-3% sulfuric acid) and is less likely to remove too much dye.