What are the characteristics of Pseudomonas aeruginosa?

What are the characteristics of Pseudomonas aeruginosa?

Pseudomonas aeruginosa is a Gram-negative, rod-shaped, asporogenous, and monoflagellated bacterium. It has a pearlescent appearance and grape-like or tortilla-like odour. P. aeruginosa grows well at 25°C to 37°C, and its ability to grow at 42°C helps distinguish it from many other Pseudomonas species.

How do you identify Pseudomonas bacteria?

They can both be distinguished from other pseudomonads by their negative oxidase reaction and production of non-diffusible yellow pigment. Primary culture for Pseudomonas species should be performed on blood agar and/or Pseudomonas selective agar.

Is Pseudomonas aeruginosa a lactose fermenter?

Panel C shows Pseudomonas aeruginosa, a lactose non-fermenter. When bacteria ferment a sugar, the pH of the medium becomes acidic. In this way, lactose-fermenting colonies were surrounded by a haze of precipitated bile.

What Colour is Pseudomonas on nutrient agar?

greenish
28.2: Pseudomonas aeruginosa on nutrient agar showing greenish colouration due to production of Pyoverdin pigment. Pseudomonas produces large, opaque, flat colonies with irregular margins and distinctively fruity odour colonies.

How would you describe the gram reaction and morphology of Pseudomonas?

Pseudomonas aeruginosa is a Gram-negative, rod-shaped, motile organism (polar flagella) which characteristically produce water-soluble pigments which diffuse through the medium. The oxidase and catalase test for P. aeruginosa are positive.

Does Pseudomonas aeruginosa ferment carbohydrates?

P. aeruginosa has few nutritional requirements and can adapt to conditions not tolerated by other organisms. It does not ferment lactose or other carbohydrates but oxidizes glucose and xylose.

Does Pseudomonas aeruginosa grow peas?

PEA agar is a selective medium that permits the growth of gram-positive cocci while inhibiting the growth of most gram-negative organisms. aeruginosa are gram negative rods and seem to be growing equally as well as the gram positive organisms.

Can gram positive ferment lactose?

Note: Gram-positive organisms are inhibited in MacConkey agar with bile salts and crystal violet, however, in a different formulation where bile salt and crystal violet are not incorporated, Gram-positive organisms also appear as lactose fermenters but are smaller in size than gram-negative ones.

Does Pseudomonas aeruginosa grow on pea agar?

What kind of broth to use for Pseudomonas aeruginosa?

P. aeruginosa grows well on LB broth, but can also utilize a wide range of compounds as sole carbon and/or nitrogen sources. To study growth on these sole nutrient sources, various defined minimal media are used to grow P. aeruginosa such as MOPS (3-(N-Morpholino) Propane-Sulfonic Acid) medium, M9, or M63.

How does Pseudomonas aeruginosa grow in liquid culture?

In liquid culture media like Trypticase soy broth or Nutrient broth, the growth of the bacterium occurs as dense turbidity in the broth medium with a surface pellicle which is further analyzed for the morphology (under the microscope), gram reaction, biochemical tests, and Pseudomonas aeruginosa specific tests.

What is the size of a Pseudomonas aeruginosa cell?

MORPHOLOGY OF PSEUDOMONAS AERUGINOSA (P. Shape – Pseudomonas aeruginosa is a Slender, rod shape (bacillus) bacterium. Size – The size of P. aeruginosa is about 1.5–3 mm × 0.5 mm (micrometer). Arrangement Of Cells – Pseudomonas aeruginosa is arranged singly or in pairs.

What kind of motility does Pseudomonas aeruginosa have?

Motility – Pseudomonas aeruginosa is an actively motile bacterium. Flagella – P. aeruginosa is a flagellated bacterium with a polar flagellum, arranged in an amphitrichous manner. Spores – The Pseudomonas aeruginosa is a non – sporing bacterium.