What enzyme catalyzes the conjugation of bilirubin?
Conjugation of bilirubin is catalyzed by bilirubin uridine diphosphate-glucuronosyltransferase (UDPGT-1),29 one of a family of enzymes that catalyze the hepatic glucuronidation of a number of endogenously produced or exogenously introduced small molecules, such as bilirubin, barbiturates, and phenols.
Is sulfanilic acid a carboxylic acid?
Sulfanilic acid (4-aminobenzenesulfonic acid) is an organic compound with the formula H3NC6H4SO3. It is an off-white solid. It is a zwitterion, which explains its high melting point. It is a common building block in organic chemistry….Sulfanilic acid.
| Names | |
|---|---|
| show SMILES | |
| Properties | |
| Chemical formula | C6H7NO3S |
| Molar mass | 173.19 |
What is the formula of Sulphanilic acid?
C6H7NO3S
Sulfanilic acid/Formula
What is the bilirubin attached to?
albumin
Bilirubin in the bloodstream is usually in a free, or unconjugated, state; it is attached to albumin, a protein, as it is transported. Once in the liver it conjugates with glucuronic acid made from the sugar glucose. It is then concentrated to about 1,000 times the strength found in blood plasma.
Which plasma protein carries bilirubin to the liver?
Serum albumin
2. Serum albumin binds bilirubin and carries it to the liver, where the newborn’s transient deficiency of the enzyme glucuronyl transferase leads to reduced bilirubin conjugation.
What is diazotization test?
Diazotization reaction mechanism generally involves the usage of nitrous acid and another acid in the treatment of aromatic amines in order to yield the diazonium salt.
How is the rate of reaction of diazotized sulfanilic acid measured?
The rate of coupling of the unconjugated bilirubin with diazotized sulfanilic acid was measured spectrophotometrically. The rate of reaction best corresponded to that calculated by means of the usual bimolecular equation.
What kind of pigment coupled with diazotized sulfanilic acid?
The indirect bilirubin was identified as that pigment which coupled with the diazotized sulfanilic acid only after the addition of alcohol, while the direct bili- rubin pigment coupled without the presence of alcohol.
How is diazonium salt prepared for sulfanilic acid?
PREPARATION OF SOLUTIONS The diazonium salt solution was prepared im- mediately before each study. A stock solution of 0.025 M sulfanilic acid was prepared by dissolving 4.33 g. of the acid in some water containing 1.325 g. sodium carbonate and diluted to 1 1. with dis- tilled water.
How is bilirubin measured in direct and indirect forms?
Bilirubin can be measured in “direct” and “indirect” forms. These forms reflect the concentration of conjugated and unconjugated levels respectively. The van den Bergh method involves the reaction of bilirubin with diazotized sulfanilic acid that forms dipyrryl azopigments and can be detected spectrophotometrically.