What is SSR PCR?
Simple sequence repeat anchored-PCR (SSR-PCR), using a single microsatellite primer, is an alternative approach which has better reproducibility due to the higher stringency conditions used (Ferreira et al., 2004). From: Toxoplasma Gondii, 2007.
What is SSR marker?
Microsatellites, otherwise called Simple sequence repeats (Ssrs) or Short Tandem Repeats (Strs), are rehashing sequences of 2-5 base sets of Dna.it is a sort of Variable Number Tandem Repeat (VNTR). Microsatellites are commonly co-prevailing. SSR markers are important in various gene studies. …
Why is ISSR a dominant marker?
Inter-simple sequence repeats (ISSRs) are regions in the genome flanked by microsatellite sequences. ISSR markers are easy to use, low-cost, and methodologically less demanding compared to other dominant markers, making it an ideal genetic marker for beginners and for organisms whose genetic information is lacking.
What is ISSR primer?
Description. ISSRs are DNA fragments of about 100-3000 bp located between adjacent, oppositely oriented microsatellite regions. ISSRs are amplified by PCR using microsatellite core sequences as primers with a few selective nucleotides as anchors into the non-repeat adjacent regions (16-18 bp).
What is SSR genotyping?
SSR genotyping involves the use of simple sequence repeats (SSRs) as DNA markers. SSRs, also called microsatellites, are a type of repetitive DNA sequence ubiquitous in most plant genomes. SSRs contain repeats of a motif sequence 1-6 bp in length.
Why we use SSR markers?
SSR markers have many advantages over the other marker systems. The first advantage is their high reproducibility, which would be the most important in genetic analysis. Also it does not require template DNA to be ultra pure. The second advantage of the SSR marker system is the polymorphic genetic information contents.
Is ISSR a dominant marker?
ISSR markers are inherited in Mendelin mode and segregated as dominant markers. This technique has been widely used in the studies of cultivar identification, genetic mapping, gene tagging,genetic diversity, evolution and molecular ecology.
What is ISSR PCR?
Inter simple sequence repeat (ISSR)-PCR is a technique that involves the use of microsatellite sequences as primers in a PDR to generate multilocus markers. Sequences amplified by ISSR-PCR can be used for DNA fingerprinting.
What is ISSR technique?
Inter simple sequence repeat (ISSR)-PCR is a technique that involves the use of microsatellite sequences as primers in a polymerase chain reaction to generate multi-locus markers.