What is BW25113?
Escherichia coli BW25113 is a common laboratory strain that was created in the laboratory of Barry L. Wanner and was utilized in a method taking advantage of the bacteriophage lambda red recombination system to perform gene disruptions with double-stranded PCR products (1).
What is E coli K12 strain?
E coli K12. E. coli K12, the prokaryotic wimp. Escherichia coli is a gram-negative bacterium that is a normal component of the human intestinal flora, and is ordinarily not a dangerous organism.
What is E coli MG1655?
MG1655 is the strain chosen by the Blattner group for the first published sequence of a wild-type laboratory strain of E. coli K-12. Although MG1655 was chosen for having few genetic manipulations from the archetypal E. coli K-12 strain, most strains used in E.
What is lacIq?
Quick Reference. A mutation in the promoter of the lacI gene of E. coli that results in increased transcription and higher levels of lac repressor within cells. From: lacIq in Oxford Dictionary of Biochemistry and Molecular Biology ยป
What is Keio collection?
coli Keio Knockout Collection was designed to provide researchers with in-frame, single-gene deletion mutants for all non-essential E. coli K-12 genes. The E. coli Keio Knockout Collection contains a set of single-gene, knockout mutants for all nonessential genes in E.
Are there good strains of E. coli?
There are plenty of good E. coli strains such as those that assist in food digestion in the human intestines. However, there are also a number of harmful E. coli strains that cause urinary tract infections, meningitis, and intestinal infections.
What does Shiga toxin do?
Shiga toxins act to inhibit protein synthesis within target cells by a mechanism similar to that of the infamous plant toxin ricin.
Why is K12 E. coli safe?
The strain E. coli K-12 is a debilitated strain which does not normally colonize the human intestine. It has also been shown to survive poorly in the environment, has a history of safe commercial use, and is not known to have adverse effects on microorganisms or plants.
Which risk group does E. coli K-12 strain belong?
Risk Group 1
E. coli K-12 is a “lab strain”. The K-12 strain cannot survive in the human digestive system, and does not produce toxins. K-12 is Risk Group 1, and it may be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.
What is the genome size of E coli mg1655?
4641652
| Entry | T00007 Complete Genome |
|---|---|
| Sequence | RS:NC_000913 (GB:U00096) |
| Length | 4641652 |
| Statistics | Number of nucleotides: 4641652 Number of protein genes: 4240 Number of RNA genes: 179 |
| Created | 1997 |
How do you read e coli genotype?
Genes: In E. coli, the genotype only includes the genes that carry a loss of function mutation. The gene name is listed as three-letters in lower case and italics (DNA methylase is written as dam). Different genes affecting the same function/pathway are identified with different uppercase italic letters.