Are Alexa Fluor tandem dyes?

Are Alexa Fluor tandem dyes?

Alexa Fluor Dye–Phycobiliprotein Tandem Conjugates.

Which BV dyes are tandem?

Examples of tandem dyes include PE-Cy7, PE-Cy5. 5, PE-Atto 594, and APC-Cy7.

Is Brilliant Violet tandem?

The seven Brilliant Violet polymer dyes come in two forms—a base polymer and a tandem polymer. These dyes are bright and in many cases, provide unprecedented brightness for dyes excited by the violet laser (Figure 1).

Is BV605 a tandem?

405 nm 610/20 BD Horizon Brilliant™ Violet 605 (BV605) (Exmax 407 nm/Emmax 602 nm) is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 602 nm. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates.

Is BV711 a tandem dye?

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm.

Is PerCP a tandem dye?

These ready-made tandems of R-PE, PerCP and APC are optimized for brightness, spill-over and photostability.

Is BUV395 a tandem dye?

BUV496, BUV563, BUV661, BUV737, and BUV805 are tandem dyes consisting of BUV395 and an acceptor dye with emission indicated by the name of the dye. The tandem dyes can be chosen based on brightness requirements, spillover into other dyes in the panel, and reagent availability.

What color is BV510?

BD Horizon™ Brilliant Violet™ 510 (BV510) (Exmax 405 nm/Emmax 510 nm) is a polymer-based dye that is brighter than BD Horizon™ V500.

What are the characteristics of a tandem dye?

In this blog, we’ll describe the common characteristics of tandem dyes, discuss their advantages in multicolor flow cytometry, and address a common myth about tandem degradation. What is a tandem dye? A tandem dye is composed of two covalently attached fluorescent molecules.

How are tandem fluorophores used in flow panels?

Without tandem fluorophores, each laser would only be suitable to discretely excite a small number of fluorophores, thus severely limiting the total number of parameters able to be detected. Table 1. Example of multicolor flow panel diversification based on available lasers, detectors, and instrument.

Can a donor dye be used with a tandem dye?

Therefore, though donor dyes and their tandems are excited by the same laser, they can be used in the same panel by utilizing different detectors to readout their emission. As an example, Figure 1 demonstrates the excitation and emission spectrum of BV421™ and its tandems.

Which is the acceptor of a tandem molecule?

A tandem is composed of two covalently attached fluorescent molecules (one of which serves as the donor and the other as acceptor) that behaves as a unique fluorophore with the excitation properties of the donor and the emission properties of the acceptor.