What is 4/12 Bis-Tris gel?

What is 4/12 Bis-Tris gel?

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions.

What is BIS-Tris gel?

NuPAGE Bis-Tris Gels and Bolt Bis-Tris Plus Gels are high-performance precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions.

What is a 4/12 gel?

A variety of 4-12% gradient gels can be found from different suppliers for protein electrophoresis. Precast gradient gels are solid, polymerized acrylamide slabs with a gradually increasing concentration toward the bottom of the gel.

What is MOPS running buffer?

MOPS-SDS Running Buffer is specially formulated to be used for running proteins on a gel. This running buffer recommended for separating small- to medium-sized proteins, provides increased resolution of small MW proteins, and shorten the electrophoresis run time.

What is NuPAGE?

Additional Information. Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications.

How do you use BIS-Tris gel?

Add 50 mL of 20X NuPAGE™ MES or MOPS SDS Running Buffer to 950 mL of deionized water to prepare 1X SDS Running Buffer. For reduced samples, add 1 mL of NuPAGE™ Antioxidant to 400 mL 1X SDS Running Buffer. a. Remove the comb, and rinse the gel wells three times using 1X Running Buffer.

What is the difference between tris and bis-Tris?

Bis-Tris gels also have a longer shelf life than Tris-Glycine gels, which begin to hydrolyze over time. Bis-Tris gels have the flexibility to be combined with either MOPS- or MES-based running buffer; the difference in migration between these two ions results in different protein separation ranges.

How do I make Bis-Tris gels?

How to Make Your Own Bis-Tris Gels

1. 5X Low MW Running Buffer. 250 mM MES. 250 mM Tris.
2. 5X High MW Running Buffer. 250 mM MOPS. 250 mM Tris.
3. 200X Running Buffer Reducing Agent. 1 M sodium bisulfite. Add to running buffer at 5mM final concentration.
4. 3.5X Gel Buffer. 1.25 M bis-Tris (pH 6.5-6.8 with HCl)

Can you reuse MOPS buffer?

So, the bottom line is: You can safely re-use the running buffer upto three times.

Can you autoclave MOPS buffer?

Bring the pH to 7.0 with NaOH (or if the MOPS sodium salt is used, then use glacial acetic acid to adjust the pH). Do not autoclave. Store in the dark, covered with foil, at room temperature.

Do precast gels have stacking?

What is the percentage of the stacking gel in your Novex™ precast protein gels? The percentage of the stacking gel is 4% in most of our gels including the Bolt™ Bis-Tris Plus gels. The NuPAGE™ Tris-Acetate gels contain a 3.2% stacking gel.

What is the difference between BIS-Tris and Tris-Glycine gels?

What kind of gel is Invitrogen bolt Bis tris plus?

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions.

How big is a nupage Bis tris gel?

NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

What kind of gel is genscript surepage Bis-tris?

GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness.

Are there any bis tris gels in MIDI format?

Certain gel chemistries (NuPAGE Bis-Tris, NuPAGE Tris-Acetate, and Invitrogen Tris-Glycine gels) are also available in the wide Midi format. Note that Bolt Bis-Tris gels are not available in the Midi format and our Thermo Scientific Precise precast gels are only available in Mini format.