What is Pfu buffer?
Description: Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. Enzyme Storage Buffer: Pfu DNA Polymerase is supplied in 50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 0.05% CHAPS and 50% glycerol.
Is PFU polymerase used in PCR?
In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step. It is a family B DNA polymerase.
What is Pfu Turbo polymerase?
1 PfuTurbo DNA polymerase is a mixture of cloned Pfu DNA polymerase and the exclusive thermostable ArchaeMaxx polymerase-enhancing factor that enhances PCR product yields and increases target length capability without altering DNA replication fidelity. PfuTurbo DNA polymerase can be used to amplify complex genomic DNA.
Is PFU polymerase thermostable?
Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium.
What PFU stands for?
A plaque-forming unit (PFU) is a measure used in virology to describe the number of virus particles capable of forming plaques per unit volume. The concept of plaque-forming units of virus is equivalent to the concept of colony-forming units of bacteria.
Why is Pfu polymerase more efficient than the Taq polymerase?
Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity, meaning that it works its way along the DNA from the 5′ end to the 3′ end and corrects nucleotide-misincorporation errors. The downside of Pfu is its speed which is slower that that of Taq.
Why is PFU more efficient than the Taq polymerase?
Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proof reading activity, meaning that it works its way along the DNA from the 5′ endto the 3′ endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts.
Is PFU a high-fidelity polymerase?
High-Fidelity PCR. Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium.
Why PFU polymerase is more efficient than Taq polymerase?
Is PFU the same as CFU?
Plaque-forming units (PFUs) are equivalent in concept to colony-forming units (CFUs) when plating bacteria, that is, where a single bacterium, bacterial arrangement, or clump of bacteria all can give rise to only a single colony upon plating.
What is the reaction buffer for Pfu DNA polymerase?
Reaction Buffer In Pfu DNA polymerase-based PCR, the reaction buffers for native and cloned Pfu DNA polymerases are formulated for optimal PCR yield and fidelity.6 Use the 10× reaction buffer provided with each polymerase for all PCR applications.
How much Pfu is needed for PCR amplification?
The concentration of Pfu DNA polymerase required for optimal PCR product yield and specificity depends on the individual target system to be amplified. Successful amplification can usually be achieved using 2.5–5.0 U of enzyme/100-l reaction for PCR targets that are <2 kb. Further optimization will be required for targets greater than 2 kb.
How big is a PfuTurbo DNA polymerase template?
The amount of PfuTurbo DNA polymerase varies depending on the length of the template to be amplified. The standard amount for vector targets up to 19 kb and genomic targets up to 6 kb in length is 1 μl (2.5 U).