What is sodium pyruvate used for?

What is sodium pyruvate used for?

What is the purpose of sodium pyruvate in media? Sodium pyruvate serves as an additional energy source for cells in culture. It is often added to low-glucose formulations (1.0 g/L glucose) and is sometimes added to higher-glucose formulations as well.

Is sodium pyruvate stable?

Storage/Stability Sterile filtered commercial solutions of sodium pyruvate are stable up to 24 months (100 mM, Product Number S 8636), when stored at 2-8 °C.

Can you freeze sodium pyruvate?

Sodium pyruvate is commonly added to cell culture media as an energy source in addition to glucose….Specifications:

Storage and Shipping Information
Do not freeze	Ok to freeze

How do 293t cells grow?

Place the cells in the 37 °C incubator with 5% CO2. 6. Gently replace medium after 24 hours with 14 mL HEK293T Culture Medium and continue to culture at 37 ºC with 5% CO2. Cells should be ready for passage or expansion to a T175 flask in ~ 2-3 days.

How does pyruvate help with weight loss?

Calcium pyruvate is said to promote weight loss by boosting fat breakdown in the body. Pyruvate is involved in the metabolic cycle to produce energy in your body, so theoretically it makes sense that taking it as a supplement could increase the amount of energy and fat burn you experience.

Does pyruvate really work?

The more pyruvate that is in the body, the faster the Kreb Cycle will work. Once the glucose and starches are broken down, the converted energy acts as a stimulant to shed excess fat. Pyruvate has been proven in clinical research to boost your metabolism and decrease your body weight when taken regularly.

Is sodium pyruvate hazardous?

Slightly toxic by ingestion and body tissue irritant. Target organs: None known. This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).

Is sodium pyruvate same as pyruvate?

Sodium pyruvate is a salt of the conjugate anion form of pyruvic acid, known as pyruvate.

What does hepes do in cell culture media?

Addition of HEPES provides supplemental buffering to cell culture medium at pH 7.2 through 7.6. The level of HEPES in cell culture media may vary from 10mM to 25mM. In GIBCO DMEM it is at a concentration of 25mM.

How fast do 293T cells grow?

HEK-293 cells see cell doubling once every 24–48 hours depending on the care. Most HEK-293T cells double every 12–20 hours depending on conditions. Our cell doubling time is typically closer to 12 hours.

How do I freeze a 293T cell?

Freezing Cells

1. Freeze cells at a density of at least 1 x 107 viable cells/ml.
2. Use a freezing medium composed of 90% complete medium and 10% DMSO. Prepare freezing medium immediately before use. Filter-sterilize the freezing medium and store at +4°C until use. Discard any remaining freezing medium after use.

What kind of Media do you need for 293T cells?

cDMEM media for 293T cells: 10% heat-inactivated FCS 1% Sodium Pyruvate 1% L-glutamine day 1: Dilution of Virus and infection to 293FT cells Plate 4×10^5 293FT cells/2 ml media/well in 5 wells. You will need 4 wells/virus plus one well for a negative control.

How to thaw 293ft cells in liquid nitrogen?

Use the following procedure to thaw 293FT cells to initiate cell culture. Thaw cells in prewarmed, complete medium without Geneticin. Remove the vial of frozen cells from liquid nitrogen and thaw quickly in a 37°C water bath.

How to support the growth of the 293ft cell line?

D-MEM already contains 4 mM L-glutamine, which is enough to support cell growth of the 293FT Cell Line. However, since L-glutamine slowly decays over time, the complete medium needs to be supplemented with 2 m L-glutamine.