What is the meaning of size of inoculum?
inoculum size is the required concentration of expected microorganism for a standard test. it is a believe to expect the standard suspension prepare is the inoculum size for a particular yield at different dilution rate for either pilot or industrial scale analysis.
What is inoculum of bacteria?
The inoculum is any part of the pathogen that can initiate infection. Thus, in fungi the inoculum may be spores (Figs. 2-3A–2-3C), sclerotia (i.e., a compact mass of mycelium), or fragments of mycelium. In bacteria, mollicutes, protozoa, viruses, and viroids, the inoculum is always whole individuals of bacteria (Fig.
What factors limit the size of a bacterial colony?
In general, as environmental conditions become less favorable, the pattern of growth in a colony becomes more complex [4]. Two of the main factors that affect bacterial growth in a laboratory are media nutrient density and media hardness, the latter being a result of agar concentration.
What are the characteristics of inoculum?
Inoculum characteristics are alkalinity and total ammonia nitrogen (TAN). SS-AD process parameters consist in feedstock-to-effluent ratio (F/E), particle size, carbon-to-nitrogen ratio (C/N) and total solids (TS). The response variable is defined as the 30-day methane yield.
How do you make a bacterial inoculum?
Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate. Drop the tip or toothpick into the liquid LB + antibiotic and swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator.
What is inoculum and infection?
Inoculation is the initial contact of a pathogen with a site of plant where infection is possible. The pathogen(s) that lands on or is otherwise brought into contact with the plant is called the inoculum. The inoculum is any part of the pathogen that can initiate infection.
What is inoculum and inoculum potential?
Garrett (1960) defined inoculum potential as the amount of energy available for the fungus to infect the host at the site of infection. The availability of nutrients affects the inoculum potential of the pathogen.
Why are bacterial colonies different in sizes?
colony size reduce means somehow its growth rate was hampered. In your experiment you r use salt but salt has a negative effect on E.coli.It dis regulate the osmotic balance of this bacterial cell after tolerance concentration may be this is the main reason of smaller colony size.
Why is the bacterial growth curve important?
The study of bacterial growth curves is important when aiming to utilize or inoculate known numbers of the bacterial isolate, for example to enhance plant growth, increase biodegradation of toxic organics, or produce antibiotics or other natural products at an industrial scale.
What is inoculum development?
The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth. …
What is the importance of inoculum?
The inoculum source had a significant impact on both growth and pH tolerance of the bacterial community in the EL soil. These differences between the EL soil inoculated with UL soil and FL soil were correlated to structural changes, as evidenced by both PLFA and DGGE analyses on the soil.
What is inoculum in pathology?
Inoculation is the pathogen or any part of the pathogen that contacts with the plant at certain site to initiate the infection process, such as spores, sclerotia, or fragments of mycelium of fungi may be fungal inoculum.
Is the amount of inoculum important to decrease the lag phase?
During this phase the bacteria grow and the size increases; but the population density is almost constant. In textbook, it is recommended to have at least 5% of inoculum to decrease the lag phase. How the increase of inoculum quantity can decrease the time needed for adaption and growth of each bacterial cells?
How big should a suspension be for bacteria growth?
For monitoring the bacterial growth, immense care should be taken on the homogeneity of the stock suspension. To maintain this, prepare a suspension (of any fixed volume, suppose 10ml) in any buffer/medium as per requirement by mixing one/two loop full of freshly grown bacteria.
What is the lag phase of a bacteria?
In bacterial physiology, lag phase is defined as the phase which is necessary for adaption of cells to new environment. During this phase the bacteria grow and the size increases; but the population density is almost constant. In textbook, it is recommended to have at least 5% of inoculum to decrease the lag phase.
What happens in the division cycle of a bacterial cell?
There are only four discrete events that occur during the division cycle of a growing rod-shaped bacterial cell. They are (1) the initiation of DNA replication, (2) the termination of DNA replication, (3) the initiation of pole formation, and (4) the completion of pole formation.