How is Acinetobacter baumannii identified?
Accurate identification of genus Acinetobacter can be performed using preliminary tests, viz., Gram stain, catalase, oxidase, and motility, which can be easily performed in any clinical laboratory. In-house PCR standardized in our study is specific and sensitive for the identification of Acinetobacter species.
What are the biochemical tests you would use for the identification of Acinetobacter baumannii?
baumannii infections in medical diagnostic laboratories of hospitals is usually carried out using phenotypic methods (including growth on MacConkey and blood agar media, and Gram staining) and differential biochemical tests, including oxidase, catalase, OF, TSI, motility, Simon Citrate, MR, VP and growth at …
Is Acinetobacter baumannii catalase-positive?
The genus Acinetobacter, as currently defined, comprises Gram-negative, strictly aerobic, non-fermenting, non-fastidious, non-motile, catalase-positive, oxidase-negative bacteria with a DNA G + C content of 39% to 47%.
What are the identifying characteristics of Acinetobacter spp?
Characteristics. Acinetobacters are short, plump rods, typically measuring 1.0–1.5 × 1.5–2.5 μm when in the logarithmic phase of growth, but they often become more coccoid in the stationary phase. They are Gram-negative but may appear Gram variable, as is typical of members of the Moraxellaceae generally.
Is A. baumannii oxidase positive?
baumannii is one of the most challenging bacterial pathogens because of its unique antibiotic resistance characteristics. The genus Acinetobacter includes non-lactose-fermenting, catalase-positive, non-motile, non-fastidious, oxidase-negative, and aerobic Gram-negative coccobacilli.
How do you get Acinetobacter baumannii?
Acinetobacter is spread by contact with a person or environment that has the bacteria. In healthcare facilities, the bacteria can spread from workers’ hands or contaminated surfaces or healthcare items.
Is Acinetobacter baumannii oxidase test?
Acinetobacter baumannii is a glucose-oxidizing nonhemolytic strain; it is oxidase negative, catalase positive and nonmotile. It can grow in MacConkey Agar that produces a purplish hue.
What shape is Acinetobacter baumannii?
Acinetobacter baumannii is an aerobic, Gram-negative, rod-shaped bacterium of the Moraxellaceae family.
What does Acinetobacter baumannii do?
Acinetobacter baumannii can cause infections in the blood, urinary tract, and lungs (pneumonia), or in wounds in other parts of the body. It can also “colonize” or live in a patient without causing infections or symptoms, especially in respiratory secretions (sputum) or open wounds.
What kills Acinetobacter baumannii?
baumannii are extensively resistant to numerous antibiotics, and the use of polymyxin antibiotics against these infections is often the final treatment option. Historically, the polymyxins have been thought to kill bacteria through membrane lysis.
What are the virulence factors of Acinetobacter baumannii?
baumannii, compared to virulence factors reported for other Gram-negative bacteria. These virulence factors, including efflux pumps, hemolytic factors, iron acquisition systems, lipopolysaccharides and OmpA, can induce host immune system responses or bacterial adherence to epithelial cells.
Is a baumannii oxidase positive?
How many isolates of Acinetobacter baumannii were identified?
All PCR positive isolates were examined to grow on CHROMagar Acinetobacter/MDR medium and their antibiotics susceptibility. A total of 335 Gram negative bacilli isolates were obtained according to the morphological characteristics and traditional biochemical test. Only 23 (6.8%) were identified as suspected A. baumannii.
Which is gold standard method to detect multi drug resistant baumannii?
The present findings suggest that the PCR using16S rRNA gene CHROMagar were gold standard method for detection of multi-drug resistant A. baumannii isolates among patients in Najaf hospitals. Content may be subject to copyright. pathogen.
Which is the most common species of Acinetobacter?
The most predominant species was Acinetobacter calcoaceticus-A. baumannii(Acb) complex (72%). High incidence of resistance was recorded for piperacillin (55%), followed by ceftriaxone (46%) and ceftazidime (46%). Isolation rate and antibiotic resistance was higher in the Intensive Care Units (ICUs) of the hospital.
Which is the most effective antibiotic for A baumannii?
Rahbar et al.[20] found high rate of resistance to A. baumannii for ceftriaxone (90.9%), piperacillin (90.9%), ceftazidime (84.1%), ciprofloxacin (90.9%) and imipenem was the most effective antibiotic, which is consistent with our observation.