What are the examples of reporter genes?
Examples of reporter genes Common reporter genes are β-galactosidase, β-glucuronidase and luciferase. Various detection methods (see below) are used to measure expressed reporter gene protein. These include luminescence, absorbance and fluorescence.
Is luciferase an assay?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. If the protein is able to upregulate transcription of the target gene, the cells will express luciferase. If the protein downregulates transcription, the cells will express less luciferase than normal.
What is gene assay?
The study of the profile of gene expression in a cell or tissue at a particular moment gives an insight into the plans of the cell for protein synthesis. These “gene expression assays” complement or replace previous assays which measured the gene expression of only one gene, or a select group of genes.
How does a reporter assay work?
A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer.
What is a luciferase reporter assay?
A luciferase reporter assay is a test that investigates whether a protein can activate or repress the expression of a target gene using luciferase as a reporter protein (Carter & Shieh, 2015). This bioluminescence directly corresponds with the effect of the protein on expression of the target gene.
Are reporters assay?
How are reporter genes used in biological assays?
Reporter assays provide a way to easily measure a biological response. There are many reporter genes available such as alkaline phosphatase, β-galactosidase, fluorescent proteins and luciferases.
How is the luciferase reporter assay used in cell biology?
This reporter assay can be used to study gene expression as well as other cellular components and events that are involved in gene regulation.
What kind of reagents are used for reporter assay?
The detection reagents have varying signal brightness and stability, include both lytic or live-cell options, and can provide either single- or dual-reporter detection.
Can a reporter gene be detected without lysis?
Some intracellular luciferases, such as NanoLuc® and Renilla luciferases, can be detected without cell lysis to create live-cell reporter assays that measure the kinetics of a cellular response. Most reporter gene assays involve either one reporter gene or two.