What are fluorescent microspheres?
Fluorescent microspheres are round spherical particles that emit bright colors when illuminated by UV light. Ability to emit intense color under UV (black light) illumination provides contrast and visibility of microspheres relative to background materials.
What is polystyrene microspheres?
Polystyrene microspheres present a flexible platform for applications in diagnostics and bioseparations. They may be coated with recognition molecules, such as antibodies, antigens, peptides, or nucleic acid probes, and can be loaded with hydrophobic dyes and other compounds.
Is polystyrene a fluorescent?
Commercially available polystyrene (PS) fluorescent microspheres are widely used in biological field for tracing, in vivo imaging and calibration of flow cytometry, among other applications.
What are silica microspheres?
Silica microspheres and nanospheres are composed of synthetic amorphous silica made in a laboratory batch environment. Additional advantage of silica microspheres is glass transition temperature of over 1000 degrees Celsius, which makes them suitable for high temperature applications.
What are polystyrene beads?
Polystyrene beads are foam beads made from heated and processed hydrocarbon. Polystyrene beads may serve as wall cavity insulation in homes and building. They are particularly effective when installed in existing walls, where it may be impractical to add traditional fiberglass blankets.
How many fluorescent dyes are in a microsphere?
Each microsphere is dyed with three different fluorescent dyes with excitation maxima of 377, 517 and 588nm, and emission maxima of 479, 546 and 612nm, respectively. These microspheres are packaged as 2.5% aqueous suspensions.
How are fluorescent microspheres used in water testing?
Specifically fluorescent microspheres are often used for water- and air-flow testing and bead-based diagnostic applications. Fluorescent microspheres are recommended for applications such as microcirculation and biological research, imaging and flow cytometry, which require the particles used to emit distinctive colors when illuminated by UV light.
How are streptavidin coated beads used in microspheres?
Polysciences’ streptavidin coated beads can be used in conjunction with a biotinylated ligand, antibody, DNA or RNA to form a bridge to the microsphere, providing the simple addition of a solid support. The streptavidin-biotin bond strength approaches that of a covalent bond, with an association constant (Ka 10^15 Mol-1) in an aqueous solution.
Which is a fluorescent monodispersed polystyrene Microsphere for covalent coupling?
Fluoresbrite® 273 Carboxylate Microspheres 1.75µm. Fluoresbrite® carboxylate microspheres are fluorescent monodispersed polystyrene microspheres that have carboxylate groups on their surfaces which can be activated for the covalent coupling of proteins.