How does miRNA inhibitor work?
The standard method for inhibiting microRNA (miRNA) function is by steric blocking, using an oligonucleotide that is perfectly complementary to the mature miRNA target. These inhibitors form a duplex with the miRNA guide strand and prevent the miRNA from binding to its intended target.
What is miR negative control?
Description. Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules.
What is miRNA mimic and inhibitor?
miRNA inhibitor is a chemically modified inhibitor of specific target miRNAs in cells. miRNA mimics are innovative molecules designed for gene silencing approaches. miRNA mimics contain nonnatural or artificial double-stranded miRNA-like RNA fragments.
How do you silence miRNA?
A widely employed approach in miRNA loss-of-function studies is to use chemically modified antisense oligonucleotides, termed antimiRs, which sequester the mature miRNA in competition with cellular target mRNAs leading to functional inhibition of the miRNA and derepression of the direct targets (Figure 1).
What is scrambled siRNA?
A scrambled control is exactly what it sounds like, it involves taking the siRNA or shRNA sequence and randomly rearranging its nucleotide sequence. A non-targeting control, on the other hand, is an siRNA/shRNA sequence designed such that it does not target any known genes in the target organism.
What is microRNA sponge?
The microRNA (miRNA) “sponge” method was introduced three years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. Sponge RNAs contain complementary binding sites to a miRNA of interest, and are produced from transgenes within cells.
How are synthetic miRNA target analogs used in medicine?
Synthetic miRNA target analogs, which are fully complementary, chemically modified oligonucleotides, have been used successfully to inhibit miRNA function. In this paper, we present a first systematic study to evaluate the effect of mismatches in the target site on synthetic inhibitor activity.
How are Target Recognition features important for miRNA inhibitors?
The data indicate that features important for natural miRNA target recognition (such as seed region complementarity) are also important for inhibitor functionality. In addition, base pairing at a second, more 3′ region appears to be equally important in determining the efficacy of synthetic inhibitors.
How are microRNAs used to control gene expression?
Micro (mi)RNAs are small (17 to 27 nucleotides), non-coding RNAs that act in association with Argonaute (Ago) proteins to modulate gene expression via an effector nucleic acid-protein complex (microribonucleoprotein (RNP) or miRNA-induced silencing complex (RISC)).
How does miRNA-based gene modulation occur in animals?
In animals, miRNA-based gene modulation occurs predominantly by the mature miRNA binding to an mRNA target site through partial base pairing, resulting in translational attenuation (for recent reviews, see [ 1 – 6 ]).