What is 5x loading dye?

What is 5x loading dye?

Description. DNA Loading Buffer Blue (5x) is a convenient ready-to-use solution to prepare samples for DNA electrophoresis. DNA Loading Buffer Blue is premixed with bromophenol blue.

How do you make 4x SDS loading dye?

To make 10 mL of 4x stock

1. 2.0 ml 1M Tris-HCl pH 6.8.
2. 0.8 g SDS.
3. 4.0 ml 100% glycerol.
4. 0.4 ml 14.7 M β-mercaptoethanol.
5. 1.0 ml 0.5 M EDTA.
6. 8 mg bromophenol Blue.

How much BME do you add to a 5x sample buffer?

Simply add 5% BME when your ready.

How do you use 5X loading dye?

For a 10 µl loading volume, add 2 µl 5x Loading Dye to 8 µl of your DNA sample, mix well and load on a gel. Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Option: Store at +4 °C for up to 6 months.

How do I make 6X Laemmli buffer?

Laemmli’s Buffer, 6x

1. 1.2g SDS (sodium dodecyl sulfate)
2. 0.01% bromophenol blue.
3. 4.7ml glycerol.
4. 1.2ml Tris 0.5M pH6.8.
5. 2.1ml ddH2O.

How do you make a 2X Laemmli buffer from 4x?

Add Reducing Agent To obtain a final 1x concentration of 355 mM 2-mercaptoethanol 2x Laemmli sample buffer: Add 50 µl of 2-mercaptoethanol per 950 µl. 4x Laemmli sample buffer: Add 100 µl of 2-mercaptoethanol per 900 µl. Alternatively, add dithiothreitol (DTT or Cleland’s reagent) to a final 1x concentration of 50 mM.

How to make SDS-PAGE protein loading buffer 5x?

2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer). 3. Boil sample for 3-5 min. 4. Allow sample to cool to room temperature. 5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.

What kind of dye is used in SDS buffer?

Can you clarify in your SDS recipe for AR1112 Buffer having the content that says the concentration of Bromophenol Blue dye used was 0.5%, whereas in the Assay Principle it says 0.05% of the same dye? Our lab technicians confirmed that SDS-PAGE Protein Loading Buffer 5X (Reducing) AR1112 contains 0.5% Bromophenol Blue dye.

How is Boster protein loading buffer 5x reducing?

Boster protein loading buffer is a 5X reducing hence it requires less sample dilution but yet accommodating more proteins load in a well. 10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, PH6.8. Upon receipt store at -20°C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.

Is the protein loading buffer 5x ( reducing ) compatible with Tris glycine?

Yes, the SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112) is compatible with tris glycine. Can you clarify in your SDS recipe for AR1112 Buffer having the content that says the concentration of Bromophenol Blue dye used was 0.5%, whereas in the Assay Principle it says 0.05% of the same dye?