What is PerCP Cy5 5?

What is PerCP Cy5 5?

5 (PerCP-Cyanine5. 5) is a photostable tandem dye excited from the 488 nm laser. It is stable in fixative, and stained cells can be stored overnight. Less-abundant intracellular antigens stained with PerCP-Cyanine5.

What color is PerCP Cy5 5?

PerCP-Cyanine® 5.5 (PerCP-Cy5. 5) is a red-emitting tandem fluorophore that combines PerCP and Cy5. 5. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.

What is PerCP in flow cytometry?

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibodies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dinoflagellates.

Are PerCP and PerCP Cy5 5 the same?

PerCP-Cy5. 5 (Ex-Max 482 nm/Em-Max 695 nm): is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5. 5 is not subject to photobleaching like PerCP and can be used with stream-in-air flow cytometers.

Can I use FITC and PE together?

Relative contribution. In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).

Does PE and FITC overlap?

In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. Fig. FITC spillover into other channels. FITC single stained lymphocytes show spillover into PE and PE-Cy5 detectors.

Is PerCP a tandem dye?

These ready-made tandems of R-PE, PerCP and APC are optimized for brightness, spill-over and photostability.

Can I use Percp and Percp Cy5 5 together?

Anyways, the two dyes cannot be used simultaneously as there is overlap in their emission spectra. And so you got the spectral overlap. As these two dyes are tandem dyes and in both of them the reciepient dye is cy5, they could not be used simultanously.

Can I use PerCP and PerCP Cy5 5 together?

Is PerCP Cy5 5 a tandem dye?

PerCP-Cy5. 5 (Ex-Max 482 nm/Em-Max 695 nm): is a tandem conjugate that combines PerCP with a cyanine dye.

Which BV dyes are tandem?

Examples of tandem dyes include PE-Cy7, PE-Cy5. 5, PE-Atto 594, and APC-Cy7.