What kind of modules are found in nidogen-2?

What kind of modules are found in nidogen-2?

Nidogen-2 contains several typical extracellular protein modules (Bork et al ., 1996), including five epidermal growth factor-like (EG) modules, two of which have a consensus sequence for calcium-binding (Campbell & Bork, 1993), two thyroglobulin type-I (TY) modules and five LDL-receptor YWTD (LY) modules (Figure 2).

What is the sequence of nidogen-2 basement membrane protein?

Human nidogen-2 was cloned and sequenced (1375 residues) and found to share 46% sequence identity and a similar domain arrangement with the previously characterized basement membrane protein nidogen-1.

Why are NID1 and NID2 genes methylated?

Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene. CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines.

Is there a biomarker for ovarian cancer nidogen 2?

Nidogen-2 is a new biomarker for ovarian cancer which correlates closely with CA125. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone

What is the AA signal sequence of nidogen-1?

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1 – 4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein.

How long does it take for nidogen-1 to adhere?

When 4 x 10 4 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.