What is HiPerFect transfection Reagent?
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations.
How does siRNA inhibit translation?
Once the single stranded siRNA (part of the RISC complex) binds to its target mRNA, it induces mRNA cleavage. This causes degradation of the mRNA and in turn no translation of the mRNA into amino acids and then proteins. Thus silencing the gene that encodes that mRNA.
What does Lipofectamine 3000 reagent do?
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
How many transfections are in a hiperfect reagent?
HiPerFect Transfection Reagent (0.5 ml) Cat. No. / ID: 301704 HiPerFect Transfection Reagent for up to 166 transfections in 24-well plates or up to 666 transfections in 96-well plates $237.00 Log in to see your account pricing. Quantity 0.5 ml 4 ml (4 x 1 ml) 1 ml 100 ml Quantity Add to cart
How does hiperfect transfection reagent work for miRNA mimics?
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. In addition to siRNA, HiPerFect Transfection Reagent is ideally suited to transfection of miRNA mimics or
Which is the most efficient transfection reagent for siRNA?
Using HiPerFect Transfection Reagent, highly efficient transfection and silencing have been observed, in some cases with as little as 10 pM siRNA. HiPerFect Transfection Reagent provides highly efficient siRNA transfection over a range of siRNA concentrations from low to high, allowing researchers to choose the siRNA concentration they wish to use.
What is the knockdown efficiency of hiperfect reagent?
Efficient knockdown (>80%) is achieved with siRNA concentrations in the range of 1–50 nM (see figure HiPerFect Reagent provides effective CDC2 knockdown ). Transfection of 1 nM siRNA resulted in 86% knockdown and transfection of 5 nM siRNA increased the knockdown efficiency to 96%.