What is the purpose of using an Enterotube II?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae.
What is the purpose of using Enterotube?
It was concluded that the Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What is the purpose of the EnteroPluri test?
EnteroPluri-Test is a 12-sector system containing special culture media that permits identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria. The system allows the simultaneous inoculation of all media present in the sectors and the execution of 15 biochemical reactions.
How is the EnteroPluri test performed?
Lay EnteroPluri-Test with its flat surface pointing upward and, by punching the plastic film, add 3 or 4 drops of Kovac’s Indole Reagent in the sector H2S/Indole. The reaction is positive if a pink-red colour develops in the added reagent within 10-15 seconds.
What are the advantages and disadvantages of EnteroPluri test?
Advantages of EnteroPluri: easier to inoculate. Disadvantages of API 20E and EnteroPluri: costly and identify only a limited number of organisms.
What is the name of the reagent utilized for indole testing?
The Indole Spot Reagent (DMACA) is suitable for anaerobe use. Since peptones have been shown to vary with regard to their suitability for use with indole testing, media selected for indole determination should be tested with known positive and negative organisms to insure suitability.
What is an EnteroPluri test quizlet?
What is the EnteroPluri Test? a multiple-test system used for rapid identification of bacteria from the family Enterobacteriaceae and other Gram-negative, oxidase negative bacteria. Each tube contains 12 compartments with the ability to run 15 tests.
What are the advantages of using EnteroPluri test kits check those that apply?
Advantages of EnteroPluri: easier to inoculate. Disadvantages of API 20E and EnteroPluri: costly and identify only a limited number of organisms. What are advantages and disadvantages of using API 20E and EnteroPluri test system in identifying unknown bacterium?
What is the clinical importance for the use of the rapid kits for the identification of enteric microorganisms?
It is clinically important to use rapid tests to treat the patient as soon as possible.
What do you need to know about the Enterotube II?
The Enterotube™ II consists of a tube with a flat side and contains 12 compartments for different biochemical tests. It has prepared sterile multimedia tube for rapid differential identification of Gram-negative bacteria (Enterobacteriaceae).
How many compartments are in Enterotube bionity 2?
It consists of a tube with a flat side and contains 12 compartments for different biochemical tests. Although the manufacturer is continuously improving the accuracy of this system, it is worth noting that this system may sometimes yield false results .
When do you read the BBL Enterotube II results?
Interpret and record all reactions with exception of indole and Voges-Proskauer. All other tests must be read before the indole and Voges-Proskauer tests are performed as the reagents added for these tests may alter the remainder of the BBL Enterotube II reactions. After 18 to 24 hours of incubation, interpret all reactions.
How is the Enterotube II colony inoculated?
The wire is sterile and need not be flamed. The Enterotube™ II is inoculated by touching the wire to a well isolated colony from a Petri plate. The wire is pulled and rotated from the other end to inoculate all the compartments and pushed all the way back in to reinoculate the compartments.