What is tergitol-7 agar?
Tergitol-7 Agar is a selective and differential medium for the detection and enumeration of coliforms in food and water samples. Tergitol-7 Agar is based on the formulation described by Chapman1 and is recommended for the selective isolation and differentiation of the coliform group.
What is TTC Agar?
General purpose relatively non-selective agar medium, containing two peptones, which will support the growth of a wide variety of organisms. Suitable for cultivation of both of aerobes and anaerobes. This medium contains the dye, 2, 3, 5- triphenyltetrazolium chloride (TTC) .
What is lactose TTC Agar?
Lactose TTC Agar with Tergitol-7 is a selective and differential medium suitable for the isolation, enumeration and identification of Coliform bacteria in food and water samples. Tergitol-7 is added to this medium to inhibit the growth of Gram-Positive bacteria and to suppress the swarming of Proteus species.
What is EC broth?
EC Broth is a selective medium for the differentiation of fecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples1,2. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique3.
What is the enrichment broth for E. coli?
An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states.
What is MacConkey broth?
MacConkey Broth is used cultivating gram negative, lactose fermenting bacilli and as a presumptive test for coliform organisms. This broth may be used for the presence of coliform organisms in food, water, milk and other materials of sanitary importance. MacConkey Broth conforms to Harmonized USP/EP/JP requirements.
What is brilliant green bile broth?
Brilliant green bile (BGB) broth is a modification of MacConkey’s liquid medium for the isolation of Enterobacteriaceae and has been formulated to attain maximum recovery of bacteria of the coli-aerogenes group, while inhibiting most gram-positive organisms that might hinder the development of the bacteria sought.
What Agar is used for E. coli?
coli/coliform Selective Agar
coli/coliform Selective Agar (formerly Chromogenic E. coli/coliform Selective Agar) is for the detection and enumeration of Escherichia coli and other coliforms from food and water samples….Dehydrated Culture Media.
| Typical Formula* | gm/litre |
|---|---|
| Agar | 10.6 |
| pH 6.7 ± 0.2 @ 25°C |
Does E coli grow on MacConkey Agar?
MacConkey agar not only selects for Gram-negative organisms by inhibiting Gram-positive organisms and yeast but also differentiates the Gram-negative organisms by lactose fermentation. Escherichia coli and other lactose ferments will produce yellow or orange colonies.
What color is a positive result in MacConkey broth?
Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli….Item No. NCM0060A.
| Specifications | |
|---|---|
| Brand | NEOGEN® Culture Media |
What is the purpose of brilliant green lactose bile broth?
Brilliant Green Bile Lactose Broth is formulated for presumptive identification and confirmation of coliform bacteria. Ox-bile and Brilliant green inhibit the growth of gram-positive bacteria including lactose fermenting Clostridia.
What media is best for growing E. coli?
‘Lysogeny’ or Luria broth (LB) is the most commonly used growth medium for E. coli. It promotes fast growth and provides good plasmid yields, making it an excellent choice for most applications, especially small-scale plasmid preps.