What is AlphaScreen?
AlphaScreen™ is a bead-based chemistry used to study biomolecular interac- tions in a microplate format. The acronym ALPHA stands for Amplified Luminescent Proximity Homogeneous Assay. As the name implies, some of the key features are that it is a non-radioactive, homogeneous proximity assay.
What does AlphaLISA stand for?
Amplified Luminescent Proximity Homogenous Assay
Discover AlphaLISA It stands for “Amplified Luminescent Proximity Homogenous Assay.” Simply, it uses two different kinds of beads, one called the “donor” and the other one called the “acceptor”, to detect the closeness of an interaction.
How does Alpha Screen work?
AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. When a biological interaction brings the beads together, a cascade of chemical reactions acts to produce a greatly amplified signal.
What is Htrf?
HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS).
What is delfia assay?
Assay principle DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.
What is Htrf used for?
Is Htrf the same as TR-FRET?
HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. HTRF assays require a microplate reader with TRF detection mode that is certified HTRF-compatible by Cisbio.
How does delfia work?
This immunoassay uses an antibody-coated plate to capture target analyte in the sample. The wells are then washed, and a DELFIA Europium-labeled antibody is added. After final washes, DELFIA Enhancement Solution is added to dissociate the Europium and allow it to form a new, highly fluorescent chelate in solution.
What is Trfia?
The time-resolved fluorescent immunoassay (TRFIA) uses a polyclonal antibody bound to immunomagnetic beads as the capture antibody and the same antibody labeled with europium as the detection antibody. Cell suspensions of 10(1) to 10(8) E. The TRFIA provides a rapid and sensitive means of detecting E.
What is HTRF technology?
What is XL665?
XL665 is a large heterohexameric edifice of 105 kDa, cross-linked after isolation for better stability and preservation of its photophysical properties in HTRF assays [6]. Unlike fluorescein, it is fully compatible with Eu cryptates.
What does delfia stand for?
DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of an analyte of interest using lanthanide chelate labeled reagents.
What kind of beads are used in alphascreen?
AlphaScreen assays are based on two types of hydrogel-coated beads, called donor and acceptor beads. The two bead types contain different chemicals that are key to the generation of the luminous signal.
What is the difference between alphascreen and alphalisa?
The hydrogel coating allows conjugation of biomolecules to the beads while reducing non-specific binding and self-aggregation. AlphaLISA is a further development of the AlphaScreen technology that relies on the same donor beads but uses a different type of acceptor beads.
What is the principle of the Alpha screen?
The fundamental principle of the AlphaScreen technology relies on binding two different molecules of interest to specific beads. In case of interaction between the two molecules and the resulting proximity of the two beads, an energy transfer from one bead to the other takes place. This results in the production of a chemiluminescent signal.
How is alphascreen used in biomolecular interactions?
AlphaScreen (Amplified Luminescent Proximity Homogeneous Assay) is a proprietary Perkin Elmer bead-based chemistry used to study biomolecular interactions. The interaction between molecules bound on the beads promotes an energy transfer from donor to acceptor beads, producing a luminescent signal.