What is Luminex used for?
Luminex xMAP (Multi-Analyte Profiling) technology enables scientists to measure multiple proteins in a single well. This technology combines advanced fluidics, optics, and digital signal processing with innovated microsphere technology to deliver multiplexed assay capabilities.
How much does Luminex cost?
Pricing for 10 or More Samples
| NUMBER OF MARKERS | 10-19 (per sample) | 59-76 (per sample) |
|---|---|---|
| 2 PLEX | $89.00 | $56.00 |
| 3 PLEX | $101.00 | $61.08 |
| 4 PLEX | $113.00 | $66.95 |
| 5 PLEX | $125.00 | $73.73 |
How much does a Magpix cost?
The average price of a Luminex MAGPIX analyzer is $27,500, only half the price of a Luminex 200 analyzer.
What is a Luminex based assay?
A Luminex assay is a type of immunoassay that precisely measures multiple analytes in one sample. The Luminex® xMAP® technology is a bead-based immunoassay that allows for multiplex detection of up to 100 analytes simultaneously.
Is Luminex an ELISA?
Enzyme-linked immunosorbent assay (ELISA) has long been the standard for quantitative analysis of metabolic hormones and cytokines. LUMINEX multiplex bead array assays were developed as cost- and time-effective alternatives to ELISA, but they are the only cost- and time-effective if they provide informative data.
How does Luminex 200 work?
Optics in the compact analyzer excite the internal dyes that identify each microsphere as well as any reporter dye captured, providing quantitation of individual analytes on each microsphere. The analyzer reads many beads from each set, enabling rapid and precise results for several targets within a single sample.
How does the Luminex 200 work?
Beads are read on a dual-laser flow-based detection instrument, such as the Luminex 200™ or FlexMap® analyzer. One laser classifies the bead and determines the analyte that is being detected. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal.
What is Magpix?
MAGPIX® is the simplest and most affordable of Luminex’s xMAP® instruments. This compact multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96-well plate in just 60 minutes. MAGPIX® features self-cleaning routines and magnetic bead compatibility (with Ma. Videos.
How does a Magpix work?
A magnet in the MAGPIX analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal.
What is luminex200?
The Luminex® 100/200™ System is a flexible analyzer based on the principles of flow cytometry that enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small samples.
How long does a Luminex assay take?
Typical timeline for sample to results for our ProcartaPlex assays is 4.5 hrs. Is it possible to measure ProcartaPlex Luminex plates on a flow cytometer? No. To analyze ProcartaPlex (Luminex) plates, you will need access to a Luminex analyzer such as a Luminex 100/200, MAGPIX, or FLEXMAP 3D system.
Is Luminex more sensitive than ELISA?
Besides the obvious advantages in terms of multiplexing ability, allowing the screening of samples for serum antibodies to multiple pathogens or antigens simultaneously in one reaction well, I found Luminex much more sensitive than ELISA specially for sample specimen with low concentration of target-analyte (antibody.
How does the Luminex high performance assay work?
Luminex Assays and High Performance Assays utilize color-coded superparamagnetic beads coated with analyte-specific antibodies. Beads recognizing different target analytes are mixed together and incubated with the sample.
What kind of beads are used in the Luminex system?
The Luminex System is the combination of three core xMAP technologies. The first is xMAP microspheres, a family of 100 fluorescently dyed 5.6 magnetic microspheres (beads) and 5.6 micron-sized polystyrene microspheres. Both bead types act as both the target identifier and the solid surface capture to build the assay.
What kind of microspheres are used in Luminex?
The Luminex System is the combination of three core xMAP technologies. The first is xMAP microspheres, a family of 100 fluorescently dyed 5.6 magnetic microspheres (beads) and 5.6 micron-sized polystyrene microspheres.
What is the function of the Luminex reader?
The Luminex reader is an essential tool that performs the key functions of this multiplex technology: The reader detects individual beads by flow cytometry. The fluidics system of the reader aligns the beads into single file as they enter a stream of sheath fluid and then enter a flow cell.