What are RAW cells?

What are RAW cells?

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. RAW-Blue™ cells express the secreted alkaline phosphatase (SEAP) reporter gene.

How do RAW cells passage?

Passage Procedure

1. Transfer RAW 264.7 growth medium 1 (RAWGM1) to a T150 flask (100 ml of growth medium per flask).
2. Place flask in incubator at 37 °C with a 5% CO2 atmosphere for at least 30 min to equilibrate the medium.
3. Enter the bar code of the cells into the cell line GUI.

What is a cell line in cell culture?

Cell line. (Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised.

How do you split a raw cell?

The average doubling time of RAW cells is 15 hours. To Split cells: Using a light microscope and if cell confluency is estimated to be around 60-70%, the cells need to be split. Warm trypsin, media and PBS for at least 20 minutes in a 36oC waterbath prior to this procedure.

Why are RAW 264.7 cells used?

The popular murine macrophage cell line, RAW 264.7, is often used to initially screen natural products for bioactivity and to predict their potential effect in vivo or on primary cells.

Are RAW cells adherent or suspension?

Subculture Routine Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask.

Are cell culture and cell line same?

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. After the first subculture, the primary culture becomes known as a cell line or subclone.

How are cell lines derived?

The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.

What is subculture ratio?

The sub-cultivation ratio is generally 1:4 to 1:6. Following manufacturers recommendations, bring total volume in each new flask up to the acceptable level with the appropriate tissue culture medium. For a 75 cm2 flask, this will usually be about 20 mL.

What kind of cells are raw 264.7 cells?

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products.

What kind of cell line is 264.7 macrophages?

RAW 264.7 macrophages. RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice.

Is the phenotypic and functional stability of Raw 264.7 cell line confirmed?

The phenotypic and functional stability of RAW 264.7 macrophages is very often discussed in literature or at various scientific discussion panels (e.g. Research Gate). However, despite RAW 264.7 cell line has been used in biological laboratories for more than 40 years, so far their stability has not yet been confirmed.

What happens to Raw 264.7 cells on LPs?

Upon LPS stimulation RAW 264.7 cells increase nitric oxide (NO) production and enhance phagocytosis. Furthermore, these cells are able to kill target cells by antibody dependent cytotoxicity [ 16 ].