What is PE Cy7?

What is PE Cy7?

PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm.

What Colour is Cy5?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What is PE fluorochrome?

PE (Ex-Max 496 nm/Em-Max 578 nm): R-phycoerythrin – is a pigment naturally found in red algae where it transfers light energy to chlorophyll during photosynthesis. PE has one of the largest absorption co-efficiencies making it one of the brightest fluorochromes available to date.

Is R PE the same as PE?

R-Phycoerythrin (also known as PE or R-PE) is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and for labeling antibodies, most often for flow cytometry.

Can I use Percp and Percp Cy5 5 together?

Anyways, the two dyes cannot be used simultaneously as there is overlap in their emission spectra. And so you got the spectral overlap. As these two dyes are tandem dyes and in both of them the reciepient dye is cy5, they could not be used simultanously.

What is Cy5 used for?

What is a Cy5 label?

Description: Cy5 PCR Labeling Kit contains all reagents (except primer, template and materials for purification of the probe) required for PCR labeling providing a highly efficient, easy-to-perform and rapid labeling technology. The kit is recommended for direct enzymatic labeling of DNA.

Can I use PerCP and PerCP Cy5 5 together?

What is PE A in flow cytometry?

PE & APC. Phycoerythrin (PE) and allophycocyanin (APC) are members of a protein family called phycobiliproteins. This family of proteins is present in photosynthetic organisms, such as algae and cyanobacteria.

What are the colors in the emission spectrum of hydrogen?

Wavelength Color 656.2 red 486.1 blue-green 434.0 blue-violet 410.1 violet Four more series of lines were discovered in the emission spectrum of hydrogen by searching the infrared spectrum at longer wave-lengths and the ultraviolet spectrum at shorter wavelengths.

Which is brighter pe-cy7 or PE conjugated reagents?

PE-Cy7 conjugated reagents are as bright as PE conjugates. However, PE-Cy7 is highly sensitive to photo-induced degradation resulting in loss of fluorescence. Care should be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative.

Which is a better conjugate PerCP or pe-cy5?

PerCP-Cy5.5 (Ex-Max 482 nm/Em-Max 695 nm): is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobleaching like PerCP and can be used with stream-in-air flow cytometers. In addition, PerCP-Cy5.5 displays less Fc-receptor mediated nonspecific staining than PE-Cy5.

What is the excitation peak of Cyanine 5?

Cy5 (Cyanine-5) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 670 nm. It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion. Cy5 (Cyanine-5) is spectrally similar to Alexa Fluor 647, iFluor 647,

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