How is Klebsiella pneumoniae identified?

How is Klebsiella pneumoniae identified?

In fact, the different members of the K. pneumoniae complex can only be reliably identified based on whole-genome sequencing (WGS) or sequencing of specific genetic markers (e.g., blaLEN, blaOKP, blaSHV,rpoB, gyrA, parC) (Haeggman et al., 2004; Brisse et al., 2014; Holt et al., 2015).

What is the biochemical test for streptococcus pneumoniae?

S. pneumoniae can be identified using Gram stain, catalase, and optochin tests simultaneously, with bile solubility as a confirmatory test. If these tests indicate that the isolate is S. pneumoniae, serological tests to identify the serotype can be performed.

What does Klebsiella pneumoniae look like?

Klebsiella pneumoniae is a Gram-negative, rod-shaped bacillus from the genus Klebsiella and family Enterobacteriaceae (Boone et al., 2001). K. pneumoniae is facultatively anaerobic, oxidase-negative, and produces acid and gas from lactose.

What type of organism is Klebsiella pneumoniae?

Klebsiella [kleb−see−ell−uh] is a type of Gram-negative bacteria that can cause different types of healthcare-associated infections, including pneumonia, bloodstream infections, wound or surgical site infections, and meningitis.

How does Enterococcus differ from Streptococcus pneumoniae?

Streptococci are gram-positive cocci arranged in pairs or chains. Streptococcus and Enterococcus species are catalase negative, which differentiates them from Staph- ylococcus, which is catalase positive. Streptococci have the typical gram-positive cell wall of peptidoglycan and teichoic acid and are nonmotile.

What are the signs and symptoms of Klebsiella pneumoniae?

Klebsiella pneumoniae symptoms

• fever.
• chills.
• coughing.
• yellow or bloody mucus.
• shortness of breath.
• chest pain.

What kind of infection is Klebsiella?

How did Klebsiella pneumoniae get its name?

Originally named Friedlander’s bacillus, it was not until 1886 when the bacterium garnered the name Klebsiella. Klebsiella pneumoniae is a gram-negative, encapsulated, non-motile bacterium found in the environment and has been associated with pneumonia in patient populations with alcohol use disorder or diabetes mellitus.

How is the serotype of S.pneumoniae determined?

S. pneumoniae can be identified using Gram stain, catalase, and optochin tests simultaneously, with. bile solubility as a confirmatory test. If these tests indicate that the isolate is S. pneumoniae, serological. tests to identify the serotype can be performed.

What kind of bacteria is Streptococcus pneumoniae?

Identification and Characterization of Streptococcus pneumoniae. S pneumoniae may occur intracellularly or extracellularly as gram-positive lanceolate diplococci, but can also occur as single cocci or in short chains of cocci. S. pneumoniae is a fastidious bacterium, growing best at 35-37°C with ~5% CO. 2 (or in a candle-jar).

What is the prognosis for Klebsiella pneumonia?

The prognosis of Klebsiella pneumonia is poor, especially in patients who are alcoholic, diabetic, have nosocomial infection or septicemia. Mortality from this type of pneumonia is above 50%.