What is the independent variable in DNA extraction?
The dependant variable of this experiment was the amount of DNA extracted, and the independent variable was the amount of each material we would use.
What is the science behind DNA extraction?
DNA extraction is a routine procedure used to isolate DNA from the nucleus of cells. When an ice-cold alcohol is added to a solution of DNA, the DNA precipitates out of solution. If there is enough DNA in the solution, you will see a stringy white mass.
Why is DNA extraction important in science?
The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity.
What is the independent variable of detergent?
In your experiment, the type of detergent used could be one independent variable. You would include a standard detergent that would serve as the control. The onion, all of the reagents, time, temperature, the concentration of detergent, and all other experimental conditions would be identical (controlled).
What are the variables in DNA extraction?
The dependent variable is the DNA. The independent variable is the different vegetables and fruits, and lats but not least the controlled variables are the amount of salt, detergent, alcohol, weight of the products, and the temperature.
What is the independent variable in the strawberry DNA lab?
The independent variable was the strawberries. The dependent variable was the amount of DNA that was extracted. Lastly, the controlled variables were the environment, mixing solution, and the types of materials.
What is the reason why DNA extraction from plant tissues is difficult?
Maintaining yield and quality of DNA during plant DNA extraction is one of the difficult tasks compared to that of animals, because of its rigid cell wall, which is made up of cellulose along with other variable levels of chemical components such as polysaccharides, polyphenols, proteins, and lipids that act as a …
What are the essential processes in DNA extraction?
The three basic steps of DNA extraction are 1) lysis, 2) precipitation, and 3) purification.
- Step 1: Lysis. In this step, the cell and the nucleus are broken open to release the DNA inside and there are two ways to do this.
- Step 2: Precipitation.
- Step 3: Purification.
What is plant DNA extraction?
DNA extraction from plant tissue can vary depending on the material used. Essentially any mechanical means of breaking down the cell wall and membranes to allow access to nuclear material, without its degradation is required.
Why would scientists want to extract the DNA out of plant cells?
Extracting the DNA from a plant is the first step in a lot of genetic studies we can do. For example, we can find out if the plant has any interesting (useful or harmful) mutations in any of its genes, which might make it better (or worse) -suited to grow in certain conditions.
What are two of the three variables in an experiment?
An experiment usually has three kinds of variables: independent, dependent, and controlled.
Why are onions used in DNA extraction experiment?
In this experiment, onions are used, this is because the onion has a low starch content, which allows the DNA to be clearly visible. Salt protects the negative end of the DNA phosphate, allowing the tip to come closer so that DNA can precipitate from a cold alcohol solution.
Why is salt used in DNA extraction experiment?
Onion DNA Extraction Experiment. Salt protects the negative end of the DNA phosphate, allowing the tip to come closer so that DNA can precipitate from a cold alcohol solution. Detergents cause cell membranes to break down by dissolving lipids and proteins from cells and disrupting bonds that hold the cell membranes together.
What happens when you put onion in liquid detergent?
Cover the chopped onion with the 100 ml of solution from step 4. The liquid detergent causes the cell membrane to break down and dissolves the lipids and proteins of the cell by disrupting the bonds that hold the cell membrane together. The detergent causes lipids and proteins to precipitate out of the solution.
What is the process of extracting DNA from a cell?
The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up.