How does Sodium citrate work as anticoagulant?
SODIUM CITRATE 4% W/V ANTICOAGULANT SOLUTION USP acts as an extracorporeal anticoagulant by binding the free calcium in the blood. Calcium is a necessary co-factor to several steps in the clotting cascade.
Why is Sodium citrate used in coagulation?
Background :Sodium citrate has been used as a coagulation test because factor V and VIII are more stable in a citrated specimen. They bind free plasma calcium to prevent clotting.
What is the physiological role of Sodium citrate?
From the medical point of view, it is used as alkalinizing agent. It works by neutralizing excess acid in the blood and urine. It has been indicated for the treatment of metabolic acidosis.
What is the mode of action of Sodium citrate?
Citrate is a weak base and so reacts with hydrochloric acid in the stomach to raise the pH. It it further metabolized to bicarbonate which then acts as a systemic alkalizing agent, raising the pH of the blood and urine 8. It also acts as a diuretic and increases the urinary excretion of calcium.
Why must Sodium Citrate be filled correctly according to its anticoagulant to blood ratio?
Fill Volume. Evacuated collection tubes must be filled to completion to ensure that a 9:1 blood-to-anticoagulant ratio is achieved. Under-filling of citrate collection tubes results in an increased anticoagulant-to-blood ratio and can extend clot-based coagulation assays.
How does sodium citrate bind to calcium?
Citrate reduces urinary saturation of calcium oxalate or calcium phosphate by forming a soluble complex with calcium and thereby reducing calcium ion activity.
What does sodium citrate do in a blood tube?
The U.S. Food and Drug Administration (FDA) is aware the U.S. is experiencing significant interruptions in the supply of sodium citrate blood specimen collection tubes, also known as light blue top tubes (hereafter “sodium citrate tubes”), used for coagulation (blood clotting) testing because of an increase in demand …
What is the pH of sodium citrate?
1. Sodium citrate solution (pH 6): 500 ml distilled water, 1.05 g sodium citrate.
How does sodium citrate prevents clotting?
Citrate is usually administered as ACD-A, but other forms are available (i.e. ACD-B and trisodium citrate), and it prevents coagulation by binding ionized calcium, which is required in clot formation.
Why Sodium citrate is not used as an anticoagulant?
Citrate and Calcium Sodium citrate anticoagulant in blood products binds ionized calcium and in high volume transfusions, especially of fresh frozen plasma, may result in transient hypocalcemia. Metabolism of citrate produces bicarbonate that may result in alkalosis and subsequent hypokalemia.
What is the effect of sodium citrate on blood?
Sodium citrate anticoagulant in blood products binds ionized calcium and in high volume transfusions, especially of fresh frozen plasma, may result in transient hypocalcemia. Metabolism of citrate produces bicarbonate that may result in alkalosis and subsequent hypokalemia.
Is sodium citrate a blood thinner?
This compound uses sodium citrate for its capacity to interfere with thrombosis. It is used when collecting plasma, the liquid in which blood cells are suspended. Sodium citrate helps to keep the blood thin while the plasma is extracted, before the cells and other solid components are returned to the donor.
What is sodium citrate used for in blood?
Citrate, in the form of sodium citrate or acid-citrate-dextrose, is used to disrupt the coagulation cascade and prevent clotting. These citrate compounds bind to the calcium in the blood.
What is the formula for citrate?
The formula of the chemical compound Citric acid is C6H8O7