What is the function of the T antigen of SV40 viral vectors?
The large and small tumor antigens (T antigens) are the major regulatory proteins encoded by SV40. Large T antigen is responsible for both viral and cellular transcriptional regulation, virion assembly, viral DNA replication, and alteration of the cell cycle.
What kind of vector is SV40?
Simian virus-40 (SV40), an icosahedral papovavirus, has recently been modified to serve as a gene delivery vector.
How SV40 can be used as vector?
SV40 plasmids (vectors) can be packaged only if their DNA is within the range of 3900 to 5300 bp. Since these small genomes do not have much dispensable DNA, it is almost impossible to construct a functional vector with any added genes to it.
What does large T antigen do?
Large T antigen also functions to initiate and maintain viral DNA replication and to activate transcription from the late promoter. As the level of the structural proteins rises, progeny virions are assembled.
What does T antigen mean?
Medical Definition of T antigen : any of several proteins that are produced by some tumorigenic DNA viruses (as simian virus 40) when they infect cells and that function in the transformation of normal cells into tumor cells or into tumor-forming cells and in the unwinding and replication of the DNA of the virus.
What does small T antigen do?
Small t antigen also activates the transcription of cellular genes, including cyclinD and cyclinA. Small t antigen-mediated activation of cyclinD transcription is indirect and requires small t antigen interaction with the cellular phosphatase pp2A.
How is SV40 transmitted?
Recent molecular biology and epidemiological studies suggest that SV40 may be contagiously transmitted in humans by horizontal infection, independently from the earlier administration of SV40-contaminated vaccines.
Is SV40 a plasmid?
PSF-SV40 – SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells.
Is SV40 a retrovirus?
SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors in animals, but most often persists as a latent infection….
| SV40 | |
|---|---|
| Specialty | Infectious disease |
What is SV40 plasmid?
PSF-SV40 – SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.
Is SV40 contagious?
What does SV40 stand for?
Simian virus 40 (SV40) is a small DNA tumor virus of monkey origin. This polyomavirus was administered to human populations mainly through contaminated polio vaccines, which were produced in naturally infected SV40 monkey cells.