What is sequence based typing?

What is sequence based typing?

Sequence-based typing involves PCR amplification and sequencing of specific HLA exons, which are known to be polymorphic, from genomic DNA. For each HLA locus both alleles are amplified and sequenced; therefore, it is not always possible to determine exactly which two alleles were responsible for sequence results.

What is HLA sequencing?

Abstract. DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes.

What is high resolution HLA typing?

High resolution A high-resolution typing result is defined as a set of alleles that encode the same protein sequence for the region of the HLA molecule called the antigen binding site and that exclude alleles that are not expressed as cell-surface proteins.

How do I interpret HLA typing results?

The typing result of the recipient is compared to those of the potential donor. Results indicate how many antigens match and how many antigen mismatches are present. “0 mismatches” indicates a high probability that the organ or tissue will not be rejected by the recipient.

What is HLA typing?

Human leukocyte antigen (HLA) typing is used to match patients and donors for bone marrow or cord blood transplants. HLA are proteins — or markers — found on most cells in your body. Your immune system uses these markers to recognize which cells belong in your body and which do not.

How many HLA types are there?

There are three general groups of HLA, they are HLA-A,HLA-B and HLA-DR. There are many different specific HLA proteins within each of these three groups.

What is the most common HLA type?

Results. The most common alleles found in Caucasians were HLA-A*02, 24, 01; HLA-B*35, 44, 51; DRB1*11, 13, 07; for Afro-Brazilians they were HLA-A*02, 03, 30; HLA-B*35, 15, 44; DRB1*13, 11, 03; and for Asians they were: HLA-A*24, 02, 26; HLA-B*40, 51, 52; DRB1*04, 15, 09.

What is a good HLA match?

Fully HLA Matched Sibling The recipient and selected sibling donor should be a 6/6 match at HLA-A, -B and -DRB1. In the absence of family typing to establish familial haplotypes, high resolution typing by DNA based methods should be performed and the recipient and donor fully matched at HLA-A, -B, -C, and -DRB1.

How do I know my HLA type?

You and potential donors will have blood drawn or will have the inside of your cheek swabbed. The blood or cheek swab is tested in a lab to figure out your HLA type. Your HLA type will be compared to potential donors to see if there is a match. Your doctor will decide which donor has the best HLA match for you.

How do I know if HLA is compatible?

How does matching happen?

1. You and potential donors will have blood drawn or will have the inside of your cheek swabbed.
2. The blood or cheek swab is tested in a lab to figure out your HLA type.
3. Your HLA type will be compared to potential donors to see if there is a match.

What does the term 10 10 HLA match mean?

Although HLA-matched sibling transplantation is still held as the “gold standard,” transplantation from HLA-A, -B, -C, -DRB1, and -DQB1-matched unrelated donors (so called 10/10) represent the first choice for patients without a suitable related donor. Keywords: 10/10; HLA-matched; Sibling; Unrelated donor.