What is non-nucleoside polymerase inhibitors?
Non-nucleoside polymerase inhibitors Non-nucleoside inhibitors bind outside the active site and target allosteric sites on the surface of the enzyme, downregulating the RdRp activity through induction of conformational changes (Fig.
How do NS5B inhibitors work?
In contrast to the nucleoside inhibitors, non-nucleoside analogue inhibitors do not compete with nucleotides nor the RNA template. Instead, by binding to RNA-dependent RNA polymerase, they inhibit polymerase activity by inhibiting conformational changes which are necessary for NS5B activity.
How does NS5B work?
NS5B is an RNA-dependent RNA polymerase, that is, it uses ssRNA (single-stranded RNA) as a template for the generation of dsRNA (double-stranded RNA). NS5B is a membrane-bound protein that contains a tail that anchors the protein to the membrane.
What is NS5A inhibitor?
What are NS5A inhibitors? Non-structural protein 5A (NS5A) inhibitors are a direct-acting antiviral agent used to treat hepatitis C virus (HCV). The NS5A protein has a substantial role in viral replication, packaging, assembly and complex interactions with cellular functions.
What is NS5A and NS5B?
The hepatitis C virus (HCV) NS5B protein contains the RNA dependent RNA polymerase (RdRp) activity that catalyzes the synthesis of the viral genome with other host and viral factors. NS5A is an HCV-encoded protein previously shown to localize to the replisome and be necessary for viral replication.
Which of the following is a NS3/4A protease inhibitor?
Two NS3-4A protease inhibitors (telaprevir and boceprevir) have been recently approved for the treatment of hepatitis C in combination with standard of care (pegylated interferon plus ribavirin).
What does NS5A stand for?
Nonstructural protein 5A (NS5A) is a zinc-binding and proline-rich hydrophilic phosphoprotein that plays a key role in Hepatitis C virus RNA replication. It appears to be a dimeric form without trans-membrane helices.
What does NS5A do HCV?
Nonstructural protein 5A (NS5A) is an essential component of the HCV replication complex. The NS5a protein interacts with other key viral products (NS4B, NS5B, RNA) and host cell proteins (cyclophilin A, kinases, etc.) to regulate viral replication and assembly.
What is NS5A RASs?
Resistance-associated substitutions (RASs) are amino acid substitutions in HCV protein sequences that result in decreased antiviral efficacy of the HCV DAAs. Among the HCV RASs, the NS5A RASs were found to effectively modify and decrease treatment response to NS5A inhibitor-containing regimens.
What is NS3 4A?
The NS3/4A protease is responsible for selective cleavage of polyproteins into individual viral proteins (NS4A, NS4B, NS5A, and NS5B).
Who makes Vosevi?
The manufacturer of Vosevi, Gilead Sciences, states that there have been cases of problems in some people who have taken the heart drug amiodarone and medicines containing sofosbuvir. Vosevi contains sofosbuvir.
What does NS3 protease do?
What kind of RNA is NS5B used for?
NS5B is an RNA-dependent RNA polymerase, that is, it uses ssRNA (single-stranded RNA) as a template for the generation of dsRNA (double-stranded RNA). NS5B is an RdRp involved in posttranslational processing, which is vital for HCV replication.
What are the drugs that bind to NS5B?
The drugs filibuvir and lomibuvir bind to the “thumb domain,” while the drugs, dasabuvir, HCV-796, and GSK5852 bind to the “palm domain” ( 40 ). This is about mutations in NS5B’s polypeptide chain. HCV has a high sequence diversity, resulting in what is called, “viral quasispecies.”
What is the role of NS5B in HCV replication?
NS5B Nucleoside Polymerase Inhibitors NS5B is an RdRp involved in posttranslational processing, which is vital for HCV replication. Nucleoside polymerase inhibitors (NPIs) are analogs of natural substrates that bind the active site of NS5B and terminate viral RNA chain generation.
How is NS5B involved in posttranslational processing?
NS5B is an RdRp involved in posttranslational processing, which is vital for HCV replication. Nucleoside polymerase inhibitors (NPIs) are analogs of natural substrates that bind the active site of NS5B and terminate viral RNA chain generation.