What does RNA Protect do?

What does RNA Protect do?

RNAprotect Reagents and Tubes provide immediate RNase inactivation and stabilization of RNA in tissues, cultures of Gram-positive and Gram-negative bacteria and sorted or cultured cells to preserve the gene expression profile and enable reproducible purification of high-quality RNA.

How does RNA purification work?

Organic extraction methods are considered the gold standard for RNA preparation. During this process, the sample is homogenized in a phenol-containing solution and the sample is then centrifuged. The upper aqueous phase is recovered and RNA is collected by alcohol precipitation and rehydration.

Does RNA protect DNA?

RNAprotect Cell Reagent also stabilizes DNA in cells. Immediate stabilization of RNA in sorted or cultured cells is generally a prerequisite for reliable gene expression analysis using microarray, real-time RT-PCR, or other nucleic acid-based technology.

What is RNAlater made of?

Actually, RNALater is just a near-saturated Ammonium Sulfate solution buffered with Sodium Citrate, and with some EDTA for metal chelation. Essentially, it causes protein aggregation (mostly reversible) when added to a tissue or cell suspension.

What can rneasy protect mini kits do for You?

RNeasy Protect Mini Kitsprovide stabilization of RNA in animal tissues and cells and purification of up to 100 µg total RNA from these sources. RNeasy Mini Kits and RNeasy Plant Mini Kits make multiple, simultaneous processing of a wide variety of biological samples possible in less than 30 minutes.

What does the rneasy protect saliva mini kit do?

The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. The RNeasy Protect Saliva Mini Kit includes the RNeasy Micro Kit, which purifies and concentrates total RNA using specialized RNeasy MinElute spin columns.

What do you need to know about rneasy protect?

The RNeasy Protect system – consisting of an RNeasy and RNAprotect bundle – provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit.

What does rneasy and rnaprotect tissue reagent do?

The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation allowing you to preserve and analyze the gene expression profile. The RNeasy and RNAprotect bundle enables isolation of high-quality RNA (see figure ” Prevention of degradation of mRNA in tissues “).