What is Hb HPLC blood test?
Hemoglobin (Hb) Electrophoresis by HPLC test measures and identifies different types of hemoglobin in the blood. A human body contains various types of hemoglobin in their blood. With age, the percentage of hemoglobin present in the body changes. Hemoglobin is the protein which is present inside the red blood cells.
What is HPLC test for thalassemia?
HPLC is a sensitive and precise method for the identification of Hb A2, Hb F and abnormal haemoglobins. It has become the method of choice for thalassaemia screening because of its speed and reliability.
Is HPLC and Hb electrophoresis same?
Haemoglobin electrophoresis provides qualitative analysis of Hbs (A, F, A2 and others), but high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE) allows quantitation of the Hb fractions.
What is HB chromatography?
High-performance liquid chromatography (HPLC) is a method commonly used for the detection of hemoglobin (Hb) variants. In addition to providing precise quantitation of Hb A2 and Hb F, the reported retention time and peak shape of a high number of hemoglobin (Hb) variants are very helpful for presumptive identification.
Why is HPLC blood test done?
The HPLC blood test expanded as a High Performance Liquid Chromatography test, is performed to screen Thalassemias and Hemoglobinopathies. This test is performed by taking the sample mixture and separating it for further diagnosis in a medical laboratory.
Why is HPLC used?
The purpose high performance liquid chromatography (HPLC) analysis of any drugs is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease.
Is HPLC a blood test?
What is HPLC method?
High-performance liquid chromatography (HPLC) is an important analytical method commonly used to separate and quantify components of liquid samples. In this technique, a solution (first phase) is pumped through a column that contains a packing of small porous particles with a second phase bound to the surface.
Why we do HPLC test?
What is Thalassemia test?
Doctors diagnose thalassemias using blood tests, including a complete blood count (CBC) and special hemoglobin tests. A CBC measures the amount of hemoglobin and the different kinds of blood cells, such as red blood cells, in a sample of blood.
Can a HPLC be used to identify HB variants?
HPLC should not be used as the sole means of identification of Hb variants, particularly for laboratories that analyze only small numbers of samples. Table 1. Summary of Hb variant retention times by HPLC.
What are the Chromatographic patterns of HPLC screening?
The HPLC chromatographic patterns were normal (Hb FA, where F is fetal and A is adult Hb) in 73,163 CBU (95.87%), and revealed an abnormal Hb in the remaining 3,149 CBU (4.13%), the majority of which were not clinically significant.
How does Hb Barts affect the HPLC chromatogram?
Thalassemia and other abnormalities. Hb Barts (γ4) elutes “Fast” in the HPLC chromatogram. Its normally low concentration in CB increases when γ-chains replace non-functional or deleted α-globin chains in α-thalassemia (α-thal).
Are there any HPLC results for sickle hemoglobin?
Among the 3,149 CBU with abnormal Hb, 87 carried two genes for Hb disorders of clinical relevance, of which 54 were homozygous for sickle hemoglobin (Hb FS), 6 for Hb C (Hb FC) and 19 were double heterozygotes (Hb FSC). The HPLC results of all CBU with Hb traits and homozygous patterns were confirmed on repeat testing.