How do you perform a manual platelet count?
Manually = Hemacytometer with whole blood measured and mixed in lysing solution specific for cell type (Unopette), counted on cytometer grid. Cell Count = (average number of cells counted x dilution factor) divided by (number of squares counted x volume of each square).
What are the direct method of manual platelet count?
The four main procedures for platelet counting are: manual phase contrast microscopy, impedance, optical light scatter/fluorescence and flow cytometry.
How is a platelet count test performed?
Most platelet tests are done on a blood sample. During the test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial.
What is the dilution for manual platelet count?
The standard dilution for platelet counts is 1:100. This dilution is prepared using the leukocyte/platelet Unopette system.
What is the diluting fluid of dry method?
Fonio (Dry Method) – Diluting fluid: 14% Magnesium Sulfate -does not lyse RBCs – Procedure: 1.
How do you count platelet slides?
Prepare and stain a smear on each specimen. For each smear, count the number of PLTs in 10 consecutive OIFs and divide by 10 to get the average number per field. Divide the automated PLT count by the average number of PLTs per field for each specimen.
What is the difference between the direct method and indirect method of platelet counting?
The methods fall into two main groups: the direct methods, in which the blood platelets are counted directly, after suitable dilution of the blood, on a haemocytometer slide; and the indirect methods, in which the proportion of platelets to erythrocytes is determined and the red cell count also, the absolute value for …
Which method of platelets counting is preferred for accurate count?
For a proper count, the platelets should be evenly distributed in the hemacytometer. Errors in platelet counting are more common when blood is collected from capillaries than from veins. Electronic counting: Electronic counting of platelets is the most common method.
Why platelet test is done?
A platelet count may be used: To screen for or diagnose various diseases and conditions that can cause problems with blood clot formation. It may be used as part of the workup of a bleeding disorder, bone marrow disease, or excessive clotting disorder, to name just a few.
What tests are done for high platelet count?
A complete blood count (CBC) measures the levels of red blood cells, white blood cells, and platelets in your blood. For this test, a small amount of blood is drawn from a blood vessel, usually in your arm. If you have thrombocythemia or thrombocytosis, the CBC results will show that your platelet count is high.
Is manual platelet count more accurate?
Manual platelet estimation using various methods in severe thrombocytopenia cases is more accurate compared to automated methods. However, the reproducibility of manual platelet counting has not been adequately studied.
What diluting fluid is used for platelet count?
Mixing is facilitated in these procedurse by the large bubble of air in the container, which is much more satisfactory. Platelet Count: Blood is diluted 1 in 20 by taking 0.1 ml of blood into 1.9 ml of diluent (R3).
How do you calculate platelet count?
Count the plateletes in all 25 squares within the one large square of the hemocytometer, counting from left to right in the first row, then left to right in the second row until all rows are complete. Multiply the number of cells counted by 1,000 to arrive at the total platelet count; for example,…
How to calculate platelet estimate?
One guideline for the estimation of platelets is as follows: Count platelets on 5 fields using 1000X magnification (care should be taken to ensure the fields used for counting are not too thick or too thin) Average the platelet counts obtained Multiply by 15 X 10 9 /L to obtain estimated platelet count (some laboratories prefer a 20 X 10 9 multiplier in this step if capillary blood is used)
How do you count platelets in a blood test?
A platelet count test measures the amount of platelets in the blood. A blood sample is taken from a vein on the inside of the elbow for the test. Because various conditions can affect the number of platelets in the blood, a platelet count test is used to indicate the presence of a disease or to determine whether there is excessive bleeding.
What causes low platelets?
If your bone marrow isn’t producing enough platelets, you’ll have a low platelet count. The causes of low platelet production include: aplastic anemia. vitamin B-12 deficiency. folate deficiency. iron deficiency. viral infections, including HIV, Epstein-Barr, and chickenpox.