What is Nfq MGB quencher?
Nonfluorescent quencher (NFQ) maximizes sensitivity The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.
Is MGB a quencher?
TaqMan MGB Probes incorporate a 5′ fluorescent reporter dye and a 3′ nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation.
What is quencher in real-time PCR?
The quencher molecule quenches the fluorescence emitted by the fluorophore when excited by the cycler’s light source via Förster resonance energy transfer (FRET). As long as the fluorophore and the quencher are in proximity, quenching inhibits any fluorescence signals.
What is Black Hole quencher?
A dark quencher (also known as a dark sucker) is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat; while a typical (fluorescent) quencher re-emits much of this energy as light.
How does a quencher work?
A quencher is very efficient at absorbing certain wavelengths. When near a dye that emits at the absorbed wavelength, the light is “quenched”, and no longer visible. Molecular beacons are effective in that the quencher actually comes in contact with the dye. …
What is reporter and quencher?
The “reporter” (R) dye is attached at the 5′-end of the probe sequence while the “quencher” (Q) dye is synthesized on the 3′-end. A popular combination of dyes is FAM or VIC for the reporter dye and TAMRA for the quencher dye.
What is BHQ quencher?
The difference between Black Hole Quencher (BHQ) dye and TAMRA. TAMRA dye is an effective quencher for fluorophores with emission maxima less than 560 nm. Dyes with longer wavelength emissions will not be effectively quenched by TAMRA.
What is ZEN quencher?
The ZEN™ quencher is a versatile modification originally developed by IDT as an internal quencher for qPCR 5′-nuclease assay fluorescence-quenched probes (Figure 1). This quencher is placed internally between the 9th and 10th base from the reporter dye on the 5′ end of a probe sequence.
What is quencher function?
A dark quencher (also known as a dark sucker) is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat; while a typical (fluorescent) quencher re-emits much of this energy as light. Dark quenchers are used in molecular biology in conjunction with fluorophores.
What is quenching agent?
Quenching refers to any process which decreases the fluorescence intensity of a given substance. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen, iodide ions and acrylamide are common chemical quenchers.
How are nonfluorescent quenchers used in TaqMan MGB probes?
Molecules that are attached to the 3′ end of TaqMan® MGB probes. When the probe is intact, the nonfluorescent quencher (NFQ) prevents the reporter dye from emitting fluorescence signal. Because the NFQ does not fluoresce, it produces lower background signals, resulting in improved precision in quantification.
What makes a MGB probe better than a NFQ?
The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data. The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.
What does the nonfluorescent quencher do to the probe?
When the probe is intact, the nonfluorescent quencher (NFQ) prevents the reporter dye from emitting fluorescence signal. Because the NFQ does not fluoresce, it produces lower background signals, resulting in improved precision in quantification.
How are MGB probes used in Applied Biosystems?
Learn more Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5′ fluorescent reporter dye and a 3′ nonfluorescent quencher (NFQ).