What are cytochemical stains used for?
Cytochemical stains are special stains used for staining peripheral blood and bone marrow smears that help in classifying and differentiating different types of leukaemias.
What does specific esterase stain?
Specific Esterase (Chloroacetate esterase) An enzymatic stain that is specific for granulocytes.
What are bone marrow assays and cytochemical stains used for?
cytochemical stains are the special stains that can be performed on peripheral blood smears and bone marrow smears. They play a very important role in diagnosis, differentiation and classification of Leukemias. 2) Immunocytochemical stains ie they identify cell specific antigens with the help of antibodies.
Which cell type is stained by both Sudan black B and peroxidase stain?
Terms in this set (90) leukemic blast stains positive w/ Sudan black B, it’s diagnostic reaction that differentiate myelogenous blast from lymphocytic blast. peroxidase stain is a good way to distinguish lymphocytic cells and its precursors vs granulocyte, partucularly immature granulocyte and its precursor.
What does Cytochemistry mean?
noun. the branch of cell biology dealing with the detection of cell constituents by means of biochemical analysis and visualization techniques.
What are cytochemical methods?
Cytochemistry is a science of localizing chemical components of cells and cell organelles on thin histological sections by using several techniques like enzyme localization, micro-incineration, micro-spectrophotometry, radioautography, cryo-electron microscopy, X-ray microanalysis by energy-dispersive X-ray …
Why PAS staining is done?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What leukemia means?
(loo-KEE-mee-uh) Cancer that starts in blood-forming tissue, such as the bone marrow, and causes large numbers of abnormal blood cells to be produced and enter the bloodstream.
What are cytochemical stains?
Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage. Nonetheless, cytochemical testing remains a useful adjunct assay for the proper classification of acute leukemia in a number of diagnostic settings.
Is Sudan Black positive in AML?
Most acute myeloid leukemia cells have strong positive reactions to myeloperoxidase and Sudan black stains.
What is SBB stain?
Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids. SBB staining is useful for for the differentiation of Acute myeloid leukemia (AML) from Acute lymphoid leukemia (ALL). It is similar to that of Myeloperoxidase (MPO) staining pattern of leukocytes and monocytes.
Which is more sensitive dual esterase or fluoride stain?
The α-naphthyl butyrate stain more specific, while the α-naphthyl acetate stain is more sensitive. In a suspected myeloid leukemia, dual esterase stains can be used as they can simultaneous classify the blasts as either myeloblasts or monoblasts. Monocytic non specific esterase is Fluoride sensitive.
Are there any specific esterases that stain monocytes?
There are two types of esterases , specific and non specific. The specific esterase is naphthol AS-D chloroacetate esterase (CAE). These identify the cells of the granulocytic series. It does not stain lymphocytes and monocytes.
How are Cytochemical stains used in bone marrow smears?
Author: Dr Faheema Hasan, MD Pathology cytochemical stains are the special stains that can be performed on peripheral blood smears and bone marrow smears. They play a very important role in diagnosis, differentiation and classification of Leukemias. These stains can be
Which is the specific esterase used to identify granulocytes?
The specific esterase is naphthol AS-D chloroacetate esterase (CAE). These identify the cells of the granulocytic series. It does not stain lymphocytes and monocytes. It can even be used to identify granulocytes in formalin fixed tissues.