What is the promoter used for expression in pGEX 4t 1?
tac promoter
pGEX vectors are widely used for GST-fusion protein expression in Escherichia coli under the control of a strong IPTG inducible tac promoter.
Is pUC19 an expression vector?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
Why is pUC19 a great cloning vector?
Why is PUC19 a great cloning vector? pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonuclease.; vector and foreign DNA cut with the enzyme.
Why does pUC19 have high copy number?
pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1.
What is pUC Ori?
It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the most widely used vector molecules as the recombinants, or the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media.
Is pUC a shuttle vector?
pUCS75, a stable high-copy-number Streptomyces–Escherichia coli shuttle vector which facilitates subcloning from pUC plasmid and M13 phage vectors. Gene. 1996 May 24;171(1):71-3. doi: 10.1016/0378-1119(96)00064-9.
Why do we use the pgex-6p expression vector?
The pGEX-6P Expression Vectors permit convenient site-specific cleavage and simultaneous purification on Glutathione Sepharose. The pGEX-6P series provides all three translational reading frames linked between the GST coding region and the multiple cloning site.
How many pGEX vectors are there in the world?
Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites.
Which is pGEX vectors provide all three translational reading frames?
Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries. 1. Kaelin, W.G. et al. Cell 70, 351 (1992).
Is the Addgene vector database a free resource?
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details.