What is non-acetylated BSA?

What is non-acetylated BSA?

UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. BSA is also a common additive for PCR amplifications, footprinting, and gel shift assays. In restriction digests, BSA has been shown to enhance enzyme activity. The molecular weight is 68 kD.

Is BSA acetylated?

BSA is available commercially in two types: either acetylated or non-acetylated.

What is BSA in buffer?

Bovine serum albumin (BSA) blocking buffer is ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications, respectively. Typically, 1-3% BSA is sufficient for most applications.

What is BSA solution used for?

In molecular biology, BSA is used to stabilize some restriction enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels.

Why is BSA acetylated?

Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. The acetylation process inactivates any trace nuclease activity that might be present.

Does BSA expire?

THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE. To effectively preserve the integrity of BSA, it should be stored at the recommended temperature and protected from light. For stability and optimal performance, this product should be stored at 2 to 8 C. Do not use after the expiration date.

What is acetylated BSA?

Description. Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. The acetylation process inactivates any trace nuclease activity that might be present.

How long can BSA in PBS be stored?

Lyophilized BSA although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution BSA should be stored at 4°C between 2-7 days and for future use below -18°C.

What kind of scopes do BSA optics use?

The BSA Optics Sweet Series Scopes use technology borrowed from military snipers to provide three ballistically calibrated turrets for different projectile weights for that caliber. ROGERS, AR (Jan. 1, 2019) – The BSA Sweet AO series uses technology applied in sniper trajectory compensation scopes in…

What does non acetylated bovine serum albumin ( BSA ) do?

Bovine Serum Albumin (BSA), non-acetylated molecular biology grade 20mg/mL Description: Highly pure non-acetylated not chemically modified BSA for use as enzyme stabiliser. Stabilises many enzymes including restriction enzymes, T4-Ligase and DNA-Polymerases during storage and incubation and reduces adhesion to reaction tubes.

How does non acetylated BSA improve PCR amplification?

Unlike acetylated BSA that inhibits PCR 1 amplification, non-acetylated BSA improves PCR amplification of low purity templates 2 and is reported to enhance PCR of GC rich templates amplification in conjunction with DMSO or formamide 3. Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 25 °C), 100mM KCl, 0.5 mM EDTA and 50 % glycerol.

When to use ultrapure BSA 50 mg / mL?

UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL.