What activates CMV promoter?
The cytomegalovirus promoter is a very potent promoter commonly used for driving the expression of transgenes, though it gradually becomes silenced in stably transfected cells. Dimethylsulfoxide and 5-Aza-2′-deoxycytidine can activate the cytomegalovirus promoter in both cell types by overlapping mechanisms.
What is the CMV promoter?
The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.
How big is the CMV promoter?
508
Plasmid: CMV promoter
| Analyze: | Sequence |
|---|---|
| Size: | 508 |
| Notes: | Human cytomegalovirus (CMV) immediate early enhancer and promoter. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
What is a CMV enhancer?
The cytomegalovirus (CMV) major immediate early (MIE) enhancer-containing promoter regulates the expression of the downstream MIE genes, which have critical roles in reactivation from latency and acute infection. The enhancer consists of binding sites for cellular transcription factors that are repeated multiple times.
Is CMV promoter constitutive expression?
A variety of cellular and viral constitutive promoters have been used in expression vectors to introduce exogenous genes into cells. These promoters have distinct advantages; for example, cytomegalovirus (CMV) early enhancer/promoter directs a high level of transient gene expression in many types of cells [1]–[4].
Where does the CMV promoter come from?
Due to strong expression and compatibility with numerous cell types, promoters derived from the enhancer region of the cytomegalovirus (CMV) immediate early (IE) promoter are among the most common for general expression vectors.
What makes a strong promoter?
The specific sequence of the promoter determines the strength of the promoter (a strong promoter leads to a high rate of transcription initiation). The presence or absence of the protein will affect the strength of the promoter.
Does CMV promoter work in humans?
Under optimal conditions, between 50%-80% transfection efficiencies could be achieved, and EGFP expression levels were maintained for at least 72 hours. Therefore, the human CMV promoter remains a useful system for transgene expression in undifferentiated ES cells.