What is the chemical formula for catalase?
2H2O2
2H2O2(aq) —-(catalase)—> 2H2O (l) + O2 (g) Figure 1. The decomposition of hydrogen peroxide to water and oxygen, as catalyzed by catalase.
What is the catalase activity equation?
Enzyme activity (Units/L) = (ΔAbs × Total assay volume) / ( Δt x ε x l x Enzyme sample volume).
What is the structure of catalase?
Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four iron-containing heme groups that allow the enzyme to react with hydrogen peroxide.
What is catalase example?
A catalase is an ubiquitous enzyme as it occurs in nearly all living organisms. As an enzyme, it catalyzes the decomposition of hydrogen peroxide into oxygen and water. Examples include peroxides, superoxides, singlet oxygen, and hydroxyl radical. …
What is the Vmax of catalase?
The value of Km for Catalase was 0.2384 and Vmax values was 13.3156 s mM / mM.
How is Vmax calculated?
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax….plotting v against v / [S] gives a straight line:
- y intercept = Vmax.
- gradient = -Km.
- x intercept = Vmax / Km.
How many amino acids are in catalase?
500 amino acids
Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four porphyrin heme (iron) groups that allow the enzyme to react with the hydrogen peroxide.
What is the function of the catalase?
Catalase is a key enzyme which uses hydrogen peroxide, a nonradical ROS, as its substrate. This enzyme is responsible for neutralization through decomposition of hydrogen peroxide, thereby maintaining an optimum level of the molecule in the cell which is also essential for cellular signaling processes.
Where can we find catalase?
liver
Found extensively in organisms that live in the presence of oxygen, catalase prevents the accumulation of and protects cellular organelles and tissues from damage by peroxide, which is continuously produced by numerous metabolic reactions. In mammals, catalase is found predominantly in the liver.
Is catalase a human?
Catalase (human) Catalase Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.