Which of the following special stains produces metachromasia in mast cells?
Metachromasia. Mast cell granules (and some other tissue components such as cartilage matrix) can naturally induce metachromatic staining. Metachromatic stains include the Romanowsky combinations (Wright, Giemsa, May-Grünwald Giemsa, and Leishman), toluidine blue, and others.
What does toluidine blue stain for?
Toluidine blue (also known as tolonium chloride) is an acidophilic metachromatic dye that selectively stains acidic tissue components (sulfates, carboxylates, and phosphate radicals). Such staining reaction is known as metachromasy and the tissue is said to exhibit metachromasia and the dye as a metachromatic dye.
What happens when mast cells are activated?
In the skin, antigens, via IgE, activate mast cells in the deep layers of connective tissue. Mast cells release histamine as well as other vasoactive molecules, which cause urticaria (hives). If the antigen activates mast cells in deeper tissue, this can lead to angioedema.
What is Metachromasia stain?
Metachromasia (var. metachromasy) is a characteristical change in the color of staining carried out in biological tissues, exhibited by certain dyes when they bind to particular substances present in these tissues, called chromotropes. The absence of color change in staining is named orthochromasia.
What is the meaning of Metachromasia?
Medical Definition of metachromasia 1 : the property of various tissues of staining in a different color (as when treatment with a blue aniline dye makes a cellular element red) 2 : the property of various biological stains that permits a single dye to stain different tissue elements in different colors.
What does mast cell activation feel?
Symptoms and Triggers of Mast Cell Activation
| MOUTH | Itching, swelling of lips and/or tongue |
|---|---|
| SKIN | Itching, hives, redness, swelling |
| GUT | Vomiting, diarrhea, cramps |
| LUNG* | Shortness of breath, cough, wheeze |
| HEART* | Weak pulse, dizziness, passing out |